Human Transaldolase ELISA Kit | TALDO1 elisa kit

Human Transaldolase ELISA Kit

Cat. Num. AAA17650

• Gene Names: TALDO1; TAL; TALH; TAL-H; TALDOR
• Reactivity: Human
• Synonyms: Transaldolase; Human Transaldolase ELISA Kit; TALDO1 (Transaldolase)/TAL/TALDOR/TALH/TAL-H; TALDO1 elisa kit

• Reactivity: Human
• Specificity: Specifically recognize TALDO1, no obvious cross reaction with other analogues
• Sequence Length: 337
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for TALDO1 elisa kit

Background: Transaldolase, contributes to the generation of NADPH in the nonoxidative phase of the pentose phosphate pathway (PPP) and is important for maintaining metabolite balance. In conjunction with several other enzymes, Transaldolase works to maintain the mitochondrial transmembrane potential by producing both ribose-5-phosphate and NADPH for use in nucleic acid and lipid biosynthesis. The role of Transaldolase in the pentose phosphate pathway of spermatoza is of significant importance, as deficiencies in Transaldolase are directly related with male infertility due to loss of sperm structure and function. Mutations in the gene encoding Transaldolase are thought to play a role in multiple sclerosis and are the direct cause of hepatosplenomegaly and telangiectases of the skin.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TALDO1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti TALDO1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TALDO1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TALDO1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 5803187
• NCBI GeneID: 6888
• NCBI Accession #: NP_006746.1
• NCBI GenBank Nucleotide #: NM_006755.1
• Molecular Weight: 37,540 Da
• NCBI Official Full Name: transaldolase
• NCBI Official Synonym Full Names: transaldolase 1
• NCBI Official Symbol: TALDO1
• NCBI Official Synonym Symbols: TAL; TALH; TAL-H; TALDOR
• NCBI Protein Information: transaldolase
• UniProt Protein Name: Transaldolase
• UniProt Gene Name: TALDO1
• UniProt Synonym Gene Names: TAL; TALDO; TALDOR
• UniProt Entry Name: TALDO_HUMAN