Rat Total Cholesterol ELISA Kit | TC elisa kit

Rat Total Cholesterol ELISA Kit

Cat. Num. AAA16126

• Reactivity: Rat
• Synonyms: Total Cholesterol; N/A; Rat Total Cholesterol ELISA Kit; TC elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHL. No significant cross-reactivity or interference between CHL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHL and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 mmol/L

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for TC elisa kit

Principle of the Assay: CHL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CHL antibody and an CHL-HRP conjugate. The assay sample and buffer are incubated together with CHL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHL concentration since CHL from samples and CHL-HRP conjugate compete for the anti-CHL antibody binding site. Since the number of sites is limited, as more sites are occupied by CHL from the sample, fewer sites are left to bind CHL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHL concentration in each sample is interpolated from this standard curve.

Intended Uses: This CHL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CHL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 452120630
• NCBI Accession #: YP_007470878.1
• NCBI GenBank Nucleotide #: NC_020307.1
• Molecular Weight: 29,822 Da
• NCBI Official Full Name: DNAse
• UniProt Protein Name: DNAse
• UniProt Entry Name: M4LLI9_SALET