SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
TDG recombinant protein
TDG, 1-410aa, Human, His tag, E Coli (Denatured)
Gene Names
TDG; hTDG
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
TDG; N/A; TDG, 1-410aa, Human, His tag, E Coli (Denatured); Thymine-DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase,; TDG recombinant protein
Host
E. coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4 urea, 10% glycerol, 0.1M NaCl
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA
Applicable Applications for TDG recombinant protein
SDS-PAGE, Denatured
Application Notes
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.
Tag Information
His-tag
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for TDG recombinant protein
Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E Coli
Product Categories/Family for TDG recombinant protein
References
um S., et al. (1998) J Biol Chem. 273:20728-20736.; Hardeland u., et al. (2002) EMBO J. 21:1456-1464.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48.4 kDa (433aa)
NCBI Official Full Name
Thymine-DNA glycosylase
NCBI Official Synonym Full Names
thymine DNA glycosylase
NCBI Official Symbol
TDG
NCBI Official Synonym Symbols
hTDG
NCBI Protein Information
G/T mismatch-specific thymine DNA glycosylase
UniProt Protein Name
G/T mismatch-specific thymine DNA glycosylase
UniProt Gene Name
TDG
UniProt Synonym Gene Names
hTDG
Similar Products
Product Notes
The TDG tdg (Catalog #AAA11810) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's TDG can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Denatured. This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined. Researchers should empirically determine the suitability of the TDG tdg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MGSMEA ENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA. It is sometimes possible for the material contained within the vial of "TDG, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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