Application Data
TFG-ALK recombinant protein
TFG-ALK Mutant, Active
Gene Names
TFG; TF6; HMSNP; SPG57; TRKT3
Applications
Kinase Assay
Synonyms
TFG-ALK; N/A; TFG-ALK Mutant, Active; TFG-ALK recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
1-193 (TFG)-1058-end (ALK)
Sequence Length
1746
Applicable Applications for TFG-ALK recombinant protein
Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for TFG-ALK recombinant protein
Description: Recombinant human fusion TFG (1-193)-ALK (1058-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Anaplastic large cell lymphomas are associated with chromosomal aberrations involving the anaplastic lymphoma kinase (ALK) gene, resulting in the expression of novel chimeric ALK proteins with transforming properties. In most of these tumors, the t (2;5) (p23;q35) generates the NPM-ALK fusion gene. Genes other than NPM have been demonstrated to fuse to the ALK gene. Two different ALK rearrangements involving the TRK-fused gene (TFG) in which the same portion of ALK was fused to different length fragments of the 5' TFG region. These two rearrangements encoded chimeric proteins of 85 kd (TFG-ALK (S)) and 97 kd (TFG-ALK (L)), respectively. TFG can provide an alternative to NPM as a fusion partner responsible for activation of the ALK and the pathogenesis of ALCL.
Scientific Background: Anaplastic large cell lymphomas are associated with chromosomal aberrations involving the anaplastic lymphoma kinase (ALK) gene, resulting in the expression of novel chimeric ALK proteins with transforming properties. In most of these tumors, the t (2;5) (p23;q35) generates the NPM-ALK fusion gene. Genes other than NPM have been demonstrated to fuse to the ALK gene. Two different ALK rearrangements involving the TRK-fused gene (TFG) in which the same portion of ALK was fused to different length fragments of the 5' TFG region. These two rearrangements encoded chimeric proteins of 85 kd (TFG-ALK (S)) and 97 kd (TFG-ALK (L)), respectively. TFG can provide an alternative to NPM as a fusion partner responsible for activation of the ALK and the pathogenesis of ALCL.
Product Categories/Family for TFG-ALK recombinant protein
References
1. Hern¡ndez L. et al: TRK-fused gene (TFG) is a new partner of ALK in anaplastic large cell lymphoma producing two structurally different TFG-ALK translocations. Blood. 1999 Nov 1;94 (9):3265-8. 2. Hern¡ndez L. et al: Diversity of genomic breakpoints in TFG-ALK translocations in anaplastic large cell lymphomas: identification of a new TFG-ALK (XL) chimeric gene with transforming activity. Am J Pathol. 2002 160 (4):1487-94. 2002.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~115 kDa
NCBI Official Full Name
Homo sapiens TRK-fused gene (TFG), transcript variant 3, mRNA
NCBI Official Synonym Full Names
TRK-fused gene
NCBI Official Symbol
TFG
NCBI Official Synonym Symbols
TF6; HMSNP; SPG57; TRKT3
NCBI Protein Information
protein TFG
UniProt Protein Name
Protein TFG
UniProt Gene Name
TFG
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Product Notes
The TFG-ALK tfg (Catalog #AAA72945) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-193 (TFG)-1058-end (ALK). AAA Biotech's TFG-ALK can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the TFG-ALK tfg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFG-ALK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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