Reactivity
Human
Specificity
The Human PP5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human PP5 proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
235
Samples
Culture Supernatants, Serum.
Sensitivity
< 10 pg/ml
Applicable Applications for TFPI2 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Related Product Information for TFPI2 elisa kit
Background: TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.
Principle of the Assay: The AAA Biotech' Human PP5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PP5 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human PP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and PP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The AAA Biotech' Human PP5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PP5 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human PP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and PP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,796 Da
NCBI Official Full Name
Tissue factor pathway inhibitor 2
NCBI Official Synonym Full Names
tissue factor pathway inhibitor 2
NCBI Official Symbol
TFPI2
NCBI Official Synonym Symbols
PP5; REF1; TFPI-2
NCBI Protein Information
tissue factor pathway inhibitor 2
UniProt Protein Name
Tissue factor pathway inhibitor 2
UniProt Gene Name
TFPI2
UniProt Synonym Gene Names
TFPI-2; PP5
UniProt Entry Name
TFPI2_HUMAN
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Product Notes
The Human TFPI2 tfpi2 (Catalog #AAA261727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261727 ELISA Kit recognizes Human TFPI2. AAA Biotech's PP5 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the TFPI2 tfpi2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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