Standard Curve (Sample)
Transforming growth factor beta 1 ELISA Kit | TGF-b1 elisa kit
Transforming growth factor beta 1 (TGF-b1)
Synonyms
Transforming growth factor beta 1; N/A; Transforming growth factor beta 1 (TGF-b1); Transforming growth factor beta 1 (TGF-beta1); TGF-b1 elisa kit
Specificity
This sandwich ELISA recognizes both natural and recombinant human TGF-beta1. The factors listed below were prepared at 50ng/mL in Calibrator Diluent I and Calibrator Diluent II and assayed for cross-reactivity. No significant cross-reactivity or interference was observed.
Sequence Length
390
Samples
Human Plasma, Serum, Milk, And Cell Culture Sample.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TGF-b1 elisa kit
Intended Uses: This human lactoferrin assay is intended for the quantitative determination of lactoferrin in human plasma, serum, milk, and cell culture sample. For research use only.
Principle of the Assay: Human lactoferrin will bind to the affinity puriffied capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human lactoferrin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human lactoferrin and is measured along with the test sample. Color development is proportional to the concentration of total lactoferrin in the samples!!Background/Introduction: Lactoferrin [ aka lactotransferrin or growth-inhibiting protein 12] is an 82.4kDa 691 amino acid glycoprotein which carries 2 ferric ions per molecule and is the main iron binding protein in milk [1]. Lactoferrin has multiple physiologcial functions including iron absorption regulation and antibacterial, antiviral, and antiparasitic activity. Degranulated neutrophils are the primaty source of plasma lactoferrin which is increased during inflammation and bacterial infection [2].
Principle of the Assay: Human lactoferrin will bind to the affinity puriffied capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human lactoferrin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human lactoferrin and is measured along with the test sample. Color development is proportional to the concentration of total lactoferrin in the samples!!Background/Introduction: Lactoferrin [ aka lactotransferrin or growth-inhibiting protein 12] is an 82.4kDa 691 amino acid glycoprotein which carries 2 ferric ions per molecule and is the main iron binding protein in milk [1]. Lactoferrin has multiple physiologcial functions including iron absorption regulation and antibacterial, antiviral, and antiparasitic activity. Degranulated neutrophils are the primaty source of plasma lactoferrin which is increased during inflammation and bacterial infection [2].
Product Categories/Family for TGF-b1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,185 Da
NCBI Official Full Name
transforming growth factor beta-1
NCBI Official Symbol
TGFB1
NCBI Protein Information
transforming growth factor beta-1; TGF-beta-1; transforming growth factor beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor-beta 1
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1
UniProt Entry Name
TGFB1_CANFA
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Product Notes
The TGF-b1 tgfb1 (Catalog #AAA77861) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Transforming growth factor beta 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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