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Application Data
(Effects of Myo/Nog cell depletion and TGF-?2 on wound healing in anterior lens explants.The wound was also covered with cells following treatment with TGF-?2 .)
TGF Beta-2 Recombinant Protein | TGFBeta-2 recombinant protein
Human TGF Beta-2 Recombinant (CHO Cell)
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Purity
>95%, as determined by SDS-PAGE and HPLC.
Synonyms
TGF Beta-2; N/A; Human TGF Beta-2 Recombinant (CHO Cell); TGFBeta-2 recombinant protein
Host
CHO cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Form/Format
Recombinant TGF-b2 is lyophilized from.2 um filtered PBS solution pH7.4
Sequence
MPPSGLRLLL LLLPLLWLLV LTPGRPAAGL STCKTIDMEL VKRKRIEAIR GQILSKLRLA SPPSQGEVPP GPLPEAVLAL YNSTRDRVAG ESAEPEPEPE ADYYAKEVTR VLMVETHNEI YDKFKQSTHS IYMFFNTSEL REAVPEPVLL SRAELRLLRL KLKVEQHVEL YQKYSNNSWR YLSNRLLAPS DSPEWLSFDV TGVVRQWLSR GGEIEGFRLS AHCSCDSRDN TLQVDINGFT TGRRGDLATI HGMNRPFLLL MATPLERAQH LQSSRHRRAL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQA LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS
Source
Optimized DNA sequence encoding Human Transforming Growth Factor Beta-2 mature chain was expressed in CHO Cells
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Function
Growth-factor, Mitogen
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months. Avoid repeated freeze/thaw cycles.
Application Data
(Effects of Myo/Nog cell depletion and TGF-?2 on wound healing in anterior lens explants.The wound was also covered with cells following treatment with TGF-?2 .)
Application Data
(Effects of Myo/Nog cell depletion and TGF-?2 on wound healing in anterior lens explants.The wound was also covered with cells following treatment with TGF-?2 .)
Application Data
(Collagen I and fibronectin protein expressions were not induced in SPARC knockdown HTFs in response to TGF-?2 but ?-SMA expression remained inducible.HTFs were treated with MMC (left panel) or transfected with siRNAs (right panel), with or without TGF-?2, for 72 hrs before being analysed for collagen I protein abundance by immunoblotting for collagen I?1 and GAPDH (loading control).)
Application Data
(Collagen I and fibronectin protein expressions were not induced in SPARC knockdown HTFs in response to TGF-?2 but ?-SMA expression remained inducible.HTFs were treated with MMC (left panel) or transfected with siRNAs (right panel), with or without TGF-?2, for 72 hrs before being analysed for collagen I protein abundance by immunoblotting for collagen I?1 and GAPDH (loading control).)
Application Data
(Influence of TGF? and TGF? inhibitors on morphology and proliferation of NHU cell cultures.(b) MTT assay showing dose-response curves of viable biomass from non-differentiated (proliferative) NHU cells treated for 4 days with TGF?1 and TGF?2.)
Application Data
(Influence of TGF? and TGF? inhibitors on morphology and proliferation of NHU cell cultures.(b) MTT assay showing dose-response curves of viable biomass from non-differentiated (proliferative) NHU cells treated for 4 days with TGF?1 and TGF?2.)
Application Data
(Treatment of microglia with IL4 increased TGF?2 expression and secretion.Quantitative RT-PCR for TGF?1 , TGF?2 and TGF?3 revealed increased TGF?2 expression after IL4 treatment.)
Application Data
(Treatment of microglia with IL4 increased TGF?2 expression and secretion.Quantitative RT-PCR for TGF?1 , TGF?2 and TGF?3 revealed increased TGF?2 expression after IL4 treatment.)
Product Categories/Family for TGFBeta-2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,341 Da
NCBI Official Full Name
transforming growth factor beta-1
NCBI Official Synonym Full Names
transforming growth factor, beta 1
NCBI Official Symbol
TGFB1
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
NCBI Protein Information
transforming growth factor beta-1; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1
UniProt Synonym Gene Names
TGFB; TGF-beta-1; LAP
UniProt Entry Name
TGFB1_HUMAN
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Product Notes
The TGFBeta-2 tgfb1 (Catalog #AAA76399) is a Recombinant Protein produced from CHO cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MPPSGLRLLL LLLPLLWLLV LTPGRPAAGL STCKTIDMEL VKRKRIEAIR GQILSKLRLA SPPSQGEVPP GPLPEAVLAL YNSTRDRVAG ESAEPEPEPE ADYYAKEVTR VLMVETHNEI YDKFKQSTHS IYMFFNTSEL REAVPEPVLL SRAELRLLRL KLKVEQHVEL YQKYSNNSWR YLSNRLLAPS DSPEWLSFDV TGVVRQWLSR GGEIEGFRLS AHCSCDSRDN TLQVDINGFT TGRRGDLATI HGMNRPFLLL MATPLERAQH LQSSRHRRAL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQA LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS. It is sometimes possible for the material contained within the vial of "TGF Beta-2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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