Standard Curve (Sample)
Mouse Tyrosine Hydroxylase ELISA Kit | TH elisa kit
Mouse Tyrosine Hydroxylase (TH) ELISA Kit
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Mouse
Synonyms
Tyrosine Hydroxylase; N/A; Mouse Tyrosine Hydroxylase (TH) ELISA Kit; TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14; TH elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of TH. No significant cross-reactivity or interference between TH and analogues was observed.
Sequence Length
106
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Competitive
Detection Range
9.88-800pg/mL. The standard curve concentrations used for the ELISA's were 800pg/mL, 266.67pg/mL, 88.89pg/mL, 29.63pg/mL, 9.88pg/mL,
Sensitivity
3.76pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TH elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of TH in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled TH and unlabeled TH (Standards or samples) with the pre-coated antibody specific to TH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of TH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of TH in the sample.
Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled TH and unlabeled TH (Standards or samples) with the pre-coated antibody specific to TH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of TH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of TH in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,898 Da
NCBI Official Full Name
tyrosine hydroxylase, partial
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
TH
NCBI Official Synonym Symbols
TYH; DYT14; DYT5b
NCBI Protein Information
tyrosine 3-monooxygenase
UniProt Protein Name
Tyrosine 3-monooxygenase
UniProt Gene Name
TH
UniProt Synonym Gene Names
TYH; TH
UniProt Entry Name
TY3H_HUMAN
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Product Notes
The Mouse TH th (Catalog #AAA144606) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA144606 ELISA Kit recognizes Mouse TH. It is sometimes possible for the material contained within the vial of "Tyrosine Hydroxylase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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