Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 ELISA Kit | TIE1 elisa kit

Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1, Tie-1 ELISA Kit

Cat. Num. AAA79964

• Gene Names: TIE1; TIE; JTK14
• Reactivity: Human
• Synonyms: tyrosine kinase with immunoglobulin-like and EGF-like domains 1; N/A; Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1, Tie-1 ELISA Kit; Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1; Tie-1 ELISA kit; RP11-282K6.6; JTK14; TIE; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 3; soluble TIE1 variant 4; soluble TIE1 variant 5; tyrosine kinase with immunoglobu; TIE1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human Tie-1. No significant cross-reactivity or interference between human Tie-1 and analogues was observed.
• Sequence Length: 1138
• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.94 ng/ml -60 ng/ml.
• Sensitivity: The minimum detectable dose of human Tie-1 is typically less than 0.23 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for TIE1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Tie-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Tie-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Tie-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Tie-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4885631
• NCBI GeneID: 7075
• NCBI Accession #: NP_005415.1
• NCBI GenBank Nucleotide #: NM_005424.4
• UniProt Accession #: P35590; B5A949; B5A950
• Molecular Weight: 125,090 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor Tie-1 isoform 1
• NCBI Official Synonym Full Names: tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• NCBI Official Symbol: TIE1
• NCBI Official Synonym Symbols: TIE; JTK14
• NCBI Protein Information: tyrosine-protein kinase receptor Tie-1; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
• UniProt Protein Name: Tyrosine-protein kinase receptor Tie-1
• UniProt Gene Name: TIE1
• UniProt Synonym Gene Names: TIE
• UniProt Entry Name: TIE1_HUMAN