Rat TIMP metallopeptidase inhibitor 1 ELISA Kit | TIMP1 elisa kit

Rat tissue inhibitors of metalloproteinase 1, TIMP-1 ELISA Kit

Cat. Num. AAA79458

• Gene Names: TIMP1; TIMP-1
• Reactivity: Rat
• Synonyms: TIMP metallopeptidase inhibitor 1; N/A; Rat tissue inhibitors of metalloproteinase 1, TIMP-1 ELISA Kit; Rat tissue inhibitors of metalloproteinase 1; TIMP-1 ELISA Kit; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinase 1; TIMP-1; TIMP1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat TIMP1. No significant cross-reactivity or interference between Rat TIMP1 and analogues was observed.
• Sequence Length: 207
• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 0.15625ng/ml-10 ng/ml
• Sensitivity: 0.224 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cardiovascular
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for TIMP1 elisa kit

Introduction: TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIMP-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TIMP-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TIMP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TIMP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 283806670
• NCBI GeneID: 100009047
• NCBI Accession #: NP_001075701.2
• NCBI GenBank Nucleotide #: NM_001082232.2
• UniProt Accession #: P20614; Q5ENG6
• Molecular Weight: 22,937 Da
• NCBI Official Full Name: metalloproteinase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: TIMP-1
• NCBI Protein Information: metalloproteinase inhibitor 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinases 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: TIMP-1
• UniProt Entry Name: TIMP1_RABIT