Transketolase 1 Recombinant Protein | tktA recombinant protein

Recombinant Escherichia coli (strain K12) Transketolase 1

Cat. Num. AAA115599

• Gene Names: tktA; ECK2930; JW5478; tkt
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Transketolase 1; N/A; Recombinant Escherichia coli (strain K12) Transketolase 1; tktA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-663aa; Full Length
• Sequence: MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for tktA recombinant protein

Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.

References

Nucleotide sequence of the Escherichia coli K-12 transketolase (tkt) gene.Sprenger G.A.Biochim. Biophys. Acta 1216:307-310(1993) Sprenger G.A.The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Escherichia coli K-12 a cooperatively developed annotation snapshot -- 2005.Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.Nucleic Acids Res. 34:1-9(2006) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli.Szumanski M.B.W., Boyle S.M.J. Bacteriol. 172:538-547(1990) Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.Mol. Cell. Proteomics 8:215-225(2009) Transketolase A of Escherichia coli K12. Purification and properties of the enzyme from recombinant strains.Sprenger G.A., Schorken U., Sprenger G., Sahm H.Eur. J. Biochem. 230:525-532(1995) Crystal structure of Escherichia coli transketolase.Isupov M.N., Rupprecht M.P., Wilson K.S., Dauter Z., Littlechild J.A.Submitted (APR-1999) to the PDB data bankStrain and near attack conformers in enzymic thiamin catalysis X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate.Asztalos P., Parthier C., Golbik R., Kleinschmidt M., Hubner G., Weiss M.S., Friedemann R., Wille G., Tittmann K.Biochemistry 46:12037-12052(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 446020759
• NCBI GeneID: 947420
• NCBI Accession #: WP_000098614.1
• NCBI GenBank Nucleotide #: WP_000098614.1
• UniProt Accession #: P27302; Q2M9Q8; Q6BF59
• Molecular Weight: 88.2 kDa
• NCBI Official Full Name: MULTISPECIES: transketolase
• NCBI Official Symbol: tktA
• NCBI Official Synonym Symbols: ECK2930; JW5478; tkt
• NCBI Protein Information: transketolase 1, thiamine triphosphate-binding
• UniProt Protein Name: Transketolase 1
• UniProt Gene Name: tktA
• UniProt Synonym Gene Names: tkt; TK 1
• UniProt Entry Name: TKT1_ECOLI