Standard Curve (Sample)
Tumor Necrosis Factor beta ELISA Kit | TNF-b elisa kit
Tumor Necrosis Factor beta (TNF-b)
Gene Names
LTA; LT; TNFB; TNFSF1
Synonyms
Tumor Necrosis Factor beta; N/A; Tumor Necrosis Factor beta (TNF-b); TNF-b elisa kit
Specificity
This assay can detect both natural and recombinant human TNF-beta. The following factors were assayed in Calibrator Diluent I for cross-reactivity. No significant crossreactivity was observed. Recombinant human: IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, GM-CSF, TGF-beta1, bFGF, TNF-alpha, TNF-beta.
Sequence Length
205
Samples
Serum, plasma, and Cell Culture Supernatant
Assay Type
Sandwich
Sensitivity
The minimum detectable quantities of human TNF-beta as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 22.0 pg/mL and 11.0 pg/mL respectively. The two standard deviations above the mean optical density of the 16 replicates of the zero standard were defined as the minimum detectable quantities.
Intra-assay Precision
To determine within-run precision, three different samples of known concentration were assayed by using 16 replicates in 1 assay.
Inter-assay Precision
To determine between-run precision, three different samples of known concentration were assayed by using replicates on 16 different assays.
Intended Uses
This Human TNF-beta ELISA kit is to be used for the in vitro quantitative determination of human Tumour Necrosis Factor Beta (TNF-beta) concentrations in serum, plasma, and cell culture supernatant. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNF-b elisa kit
Principle of the assay: This TNF-beta enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to TNF-beta. Standards or samples are then added to the appropriate microtiter plate wells and incubated. After washing to remove unbound TNF-beta and other components of the sample, biotin-conjugated polyclonal antibody specific to TNF-beta is added and incubated. If present, TNF-beta will bind and become immobilized by the antibody pre-coated on the wells and then become "sandwiched" by the biotin conjugate. In order to quantitatively determine the amount of TNF-beta present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNF-beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. This kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing) in order to measure the concentration of TNF-beta in the samples. According to the testing system, the standard provided is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus TNF-beta concentration (pg/mL). The concentration of TNF-beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TNF-b elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,297 Da
NCBI Official Full Name
tumor necrosis factor-beta
NCBI Official Synonym Full Names
lymphotoxin alpha
NCBI Official Symbol
LTA
NCBI Official Synonym Symbols
LT; TNFB; TNFSF1
NCBI Protein Information
lymphotoxin-alpha; LT-alpha; TNF superfamily, member 1; TNF-beta; tumor necrosis factor beta; tumor necrosis factor ligand superfamily member 1
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
LTA
UniProt Entry Name
TNFB_HUMAN
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Product Notes
The TNF-b lta (Catalog #AAA77863) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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