TRAIL/TNFSF10 Recombinant Protein | TNFSF10 recombinant protein

TRAIL/TNFSF10 Protein, Human, Recombinant

Cat. Num. AAA257880

• Gene Names: TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TRAIL/TNFSF10; N/A; TRAIL/TNFSF10 Protein, Human, Recombinant; Human TNFSF10/TRAIL/APO-2L/CD253 Protein; Apo-2L Protein, Human; APO2L Protein, Human; CD253 Protein, Human; TL2 Protein, Human; TRAIL Protein, Human; tumor necrosis factor (ligand) superfamily, member 10; TNFSF10 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50 mM Tris, 300 M NaCl, pH7.2. 5% trehalose, 5% mannitol and 0.01% Tween80
• Sequence: Val114-Gly281

• Source: Human
• Predicted N Terminal: Met
• Protein Construction: A DNA sequence encoding the human TNFSF10 (NP_003801.1)(Val 114-Gly 281) with an initial Met was expressed and purified.
• Reconstitution: Reconstituted with sterile deionized water to 0.25 mg/ml
• Molecular Mass: The recombinant human TNSF10 consists of 169 amino acids and has a calculated molecular mass of 19.6 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions
• Bio Activity: Loaded Recombinant Human DR5/TRAIL R2 Protein, hFc Tag (Cat. No. MBS8120286) on AHC Biosensor, can bind Recombinant Human TRAIL/TNFSF10 Protein (Cat. No. MBS8120252) with an affinity constant of 0.253 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for TNFSF10 recombinant protein

Background: Tumor necrosis factor ligand superfamily member 10 (TNFSF10), also known as TNF-related apoptosis-inducing ligand (TRAIL), Apo-2 ligand, and CD253, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TNFSF10/Apo-2L/CD253 functions as a ligand that induces the process of cell death called apoptosis. TNFSF10/TRAIL shows homology to other members of the tumor necrosis factor superfamily. As one member of the cluster of differentiation system, TNFSF10/CD253 is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion TNFSF10/Apo-2L/CD253/TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TNFSF10/TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for TNFSF10 recombinant protein

Cancer Drug Targets; Death Receptor Signaling

References

Song C, et al. (2005) TRAIL (CD253), a new member of the TNF superfamily. J Biol Regul Homeost Agents. 19(1-2): 73-7.
Kuribayashi K, et al. (2008) TNFSF10 (TRAIL), a p53 target gene that mediates p53-dependent cell death. Cancer Biol Ther. 7(12): 2034-8.
Wiley SR, et al. (1995) Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 3(6): 673-82.

NCBI and Uniprot Product Information

• NCBI GI #: 4507593
• NCBI GeneID: 8743
• NCBI Accession #: NP_003801.1
• NCBI GenBank Nucleotide #: NM_003810.3
• UniProt Accession #: P50591; A1Y9B3
• Molecular Weight: Predicted Molecular Mass: 19.63 kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: TNFSF10
• NCBI Official Synonym Symbols: TL2; APO2L; CD253; TRAIL; Apo-2L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• UniProt Gene Name: TNFSF10
• UniProt Synonym Gene Names: APO2L; TRAIL; Apo-2L
• UniProt Entry Name: TNF10_HUMAN