SDS-PAGE
(Fig. SDS-PAGE analysis of Human sRANKL protein.)
sRANKL recombinant protein
Human sRANKL protein
Gene Names
TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2
Purity
>98 % as determined by SDS-PAGE
Synonyms
sRANKL; N/A; Human sRANKL protein; soluble Receptor Activator of NF-kappaB Ligand; TNFSF11; TRANCE (TNF-related activation-induced cytokine); OPGL; ODF (Osteoclast differentiation factor); sRANKL recombinant protein
Host
E Coli
Purity/Purification
>98 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20 mM Tris, 150 mM NaCl pH 8.0.
Reconstitution
Use the buffer we provided to reconstitute the lyophilized Human sRANKL protein
Sequence
Amino acid sequence derived from Human sRANKL isoform (O14788) (Glu143-Asp317) was expressed.
Protein length
The recombinant Human sRANKL consists of 175 amino acids(N-Met) and has a predicted molecular mass of 19 kDa. The apparent molecular mass of the Human sRANKL protein is approximately 19 kDa in SDS-PAGE under reducing conditions.
Biological Activity
Immobilized human sRANKL protein at 1ug/mL (100ul/well) can bind Human OPG (C-Fc), The EC50 of Human OPG is 188 ng/mL.
Endotoxin
<0.1 EU per ug of the protein as determined by the LAL method.
Usage notes
Always centrifuge tubes before opening. It is recommended to centrifuge at 12,000 rpm for 1 minute. It is recommended to reconstitute the lyophilized Human sRANKL protein using the buffer we provided not less than 100 ug/mL, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Lyophilized Human sRANKL protein product should be stored desiccated below -18 degree C for 12 months.
Upon reconstitution, the protein should be stored at 4 degree C between 2 -7 days and below -18°C for 3 months.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Upon reconstitution, the protein should be stored at 4 degree C between 2 -7 days and below -18°C for 3 months.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
SDS-PAGE
(Fig. SDS-PAGE analysis of Human sRANKL protein.)
SDS-PAGE
(Fig. SDS-PAGE analysis of Human sRANKL protein.)
Related Product Information for sRANKL recombinant protein
Description: Human sRANKL protein, expressed in E. coli
Background: Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factorkappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is expressed in a variety of cells,including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoyreceptor called OPG. TNFSF11 is involved in a number of fundamental biological processes such as acting as regulator ofinteractions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancingbone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cellproliferation.
Background: Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factorkappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is expressed in a variety of cells,including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoyreceptor called OPG. TNFSF11 is involved in a number of fundamental biological processes such as acting as regulator ofinteractions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancingbone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cellproliferation.
Product Categories/Family for sRANKL recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 11 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 11
NCBI Official Symbol
TNFSF11
NCBI Official Synonym Symbols
ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2
NCBI Protein Information
tumor necrosis factor ligand superfamily member 11; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligan
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 11
UniProt Gene Name
TNFSF11
UniProt Synonym Gene Names
OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE
UniProt Entry Name
TNF11_HUMAN
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Product Notes
The sRANKL tnfsf11 (Catalog #AAA332526) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "sRANKL, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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