SDS-PAGE
DNMT2 Recombinant Protein | TRDMT1 recombinant protein
DNMT2 Protein, Human, Recombinant (GST Tag)
Gene Names
TRDMT1; DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
Purity
>94% as determined by SDS-PAGE
Synonyms
DNMT2; N/A; DNMT2 Protein, Human, Recombinant (GST Tag); Human DNMT2/TRDMT1 Protein (GST Tag); DMNT2 Protein, Human; DNMT2 Protein, Human; MHSAIIP Protein, Human; PUMET Protein, Human; RNMT1 Protein, Human; tRNA aspartic acid methyltransferase 1; TRDMT1 recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
>94% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, pH8.0 5% mannitol, 0.01% Tween-80
Sequence
Met1-Glu391
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Met
Tag
N-GST
Protein Construction
A DNA sequence encoding the human TRDMT1 isoform a (NP_004403.1)(Met 1-Glu 391) was fused with the GST tag at the N-terminus.
Reconstitution
It is recommended that sterile water (800ul) be added to the vial to prepare a stock solution of 0.125mg/mL. Concentration is measured by UV-Vis.
Preparation and Storage
Store it under sterile conditions at -20°C to -80°C upon receiving. The lab recommends to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles
SDS-PAGE
SDS-PAGE
Related Product Information for TRDMT1 recombinant protein
Background: DNMT2, also known as tRNA (cytosine-5-)-methyltransferase, DNA methyltransferase homolog HsaIIP, and TRDMT1, is a member of the DNA methyltransferase family of enzymes. DNMT2 enzymes have been widely conserved during evolution and contain all of the signature motifs of DNA (cytosine-5)-methyltransferases. It contains all 10 sequence motifs that are conserved among m(5)C MTases, including the consensus S:-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide, and its structure is very similar to prokaryotic DNA methyltransferases. DNMT2 has close homologs in plants, insects and Schizosaccharomyces pombe, but no related sequence can be found in the genomes of Saccharomyces cerevisiae or Caenorhabditis elegans. While the biological function of DNMT2 is not yet known, the strong binding to DNA suggests that DNMT2 may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. However, the DNA methyltransferase activity of these proteins is comparatively weak and their biochemical and functional properties remain enigmatic. Recent evidence now shows that Dnmt2 has a novel tRNA methyltransferase activity, raising the possibility that the biological roles of these proteins might be broader than previously thought.
Product Categories/Family for TRDMT1 recombinant protein
References
Dong A, et al. (2001) Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA. Nucleic Acids Res. 29(2): 439-48.Hermann A, et al. (2003) The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity. J Biol Chem. 278(34): 31717-21.Jeltsch A, et al. (2006) Two substrates are better than one: dual specificities for Dnmt2 methyltransferases. Trends Biochem Sci. 31(6): 306-8.Schaefer M, et al. (2010) Solving the Dnmt2 enigma. Chromosoma. 119(1): 35-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,087 Da
NCBI Official Full Name
tRNA (cytosine(38)-C(5))-methyltransferase
NCBI Official Synonym Full Names
tRNA aspartic acid methyltransferase 1
NCBI Official Symbol
TRDMT1
NCBI Official Synonym Symbols
DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
NCBI Protein Information
tRNA (cytosine(38)-C(5))-methyltransferase; tRNA (cytosine(38)-C(5))-methyltransferase; DNA (cytosine-5)-methyltransferase-like protein 2; DNA MTase homolog HsaIIP; DNA methyltransferase-2; tRNA (cytosine-5-)-methyltransferase
UniProt Protein Name
tRNA (cytosine(38)-C(5))-methyltransferase
UniProt Gene Name
TRDMT1
UniProt Synonym Gene Names
DNMT2; Dnmt2; DNA MTase homolog HsaIIP; M.HsaIIP
UniProt Entry Name
TRDMT_HUMAN
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Product Notes
The TRDMT1 trdmt1 (Catalog #AAA257971) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Glu39 1. It is sometimes possible for the material contained within the vial of "DNMT2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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