Thyrotropin receptor Recombinant Protein | TSHR recombinant protein

Recombinant human Thyrotropin receptor

Cat. Num. AAA18601

• Gene Names: TSHR; LGR3; CHNG1; hTSHR-I
• Applications: Calibrator or standard
• Purity: 85%±5% by SDS-PAGE
• Synonyms: Thyrotropin receptor; N/A; Recombinant human Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R; TSHR recombinant protein

• Host: E.coli
• Purity/Purification: 85%±5% by SDS-PAGE
• Form/Format: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 50% glycerol
• Sequence Positions: 21-413aa
• Sequence: GMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNP

• Applicable Applications for TSHR recombinant protein: Calibrator or standard
• Tag Info: his-tag
• Expression Region: 21-413aa
• Appearance: Clear Solution
• Preparation and Storage: Aliquot and store at < -20°C. Avoid repeated freeze/thaw cycles.

SDS-PAGE

(Recombinant human Thyrotropin receptorHis-taggedYeast derived)

Product Categories/Family for TSHR recombinant protein

Neuroscience; Raw Materials Assay Development

References

Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor.Nagayama Y., Kaufman K.D., Seto P., Rapoport B.Biochem. Biophys. Res. Commun. 165:1184-1190(1989) Cloning, sequencing and expression of the human thyrotropin (TSH) receptor evidence for binding of autoantibodies.Libert F., Lefort A., Gerard C., Parmentier M., Perret J., Ludgate M., Dumont J.E., Vassart G.Biochem. Biophys. Res. Commun. 165:1250-1255(1989) Cloning, sequencing and expression of human TSH receptor.Misrahi M., Loosfelt H., Atger M., Sar S., Guiochon-Mantel A., Milgrom E.Biochem. Biophys. Res. Commun. 166:394-403(1990) Isolation of TSH and LH/CG receptor cDNAs from human thyroid regulation by tissue specific splicing.Frazier A.L., Robbins L.S., Stork P.J., Sprengel R., Segaloff D.L., Cone R.D.Mol. Endocrinol. 4:1264-1276(1990) Cloning and sequencing of a 1.3 KB variant of human thyrotropin receptor mRNA lacking the transmembrane domain.Graves P.N., Tomer Y., Davies T.F.Biochem. Biophys. Res. Commun. 187:1135-1143(1992) Molecular cloning and sequencing of an alternatively spliced form of the human thyrotropin receptor transcript.Takeshita A., Nagayama Y., Fujiyama K., Yokoyama N., Namba H., Yamashita S., Izumi M., Nagataki S.Biochem. Biophys. Res. Commun. 188:1214-1219(1992) cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org) .Kopatz S.A., Aronstam R.S., Sharma S.V. The DNA sequence and analysis of human chromosome 14.Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.Nature 421:601-607(2003) Purification and characterization of a soluble bioactive amino-terminal extracellular domain of the human thyrotropin receptor.Cornelis S., Uttenweiler-Joseph S., Panneels V., Vassart G., Costagliola S.Biochemistry 40:9860-9869(2001) Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone receptor.Nakabayashi K., Matsumi H., Bhalla A., Bae J., Mosselman S., Hsu S.Y., Hsueh A.J.W.J. Clin. Invest. 109:1445-1452(2002) Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway.Lahuna O., Quellari M., Achard C., Nola S., Meduri G., Navarro C., Vitale N., Borg J.-P., Misrahi M.EMBO J. 24:1364-1374(2005) Structural predictions for the ligand-binding region of glycoprotein hormone receptors and the nature of hormone-receptor interactions.Jiang X., Dreano M., Buckler D.R., Cheng S., Ythier A., Wu H., Hendrickson W.A., el Tayar N.Structure 3:1341-1353(1995) Crystal structure of the TSH receptor in complex with a thyroid-stimulating autoantibody.Sanders J., Chirgadze D.Y., Sanders P., Baker S., Sullivan A., Bhardwaja A., Bolton J., Reeve M., Nakatake N., Evans M., Richards T., Powell M., Miguel R.N., Blundell T.L., Furmaniak J., Smith B.R.Thyroid 17:395-410(2007) Further studies of genetic susceptibility to Graves' disease in a Russian population.Chistiakov D.A., Savost'anov K.V., Turakulov R.I., Petunina N., Balabolkin M.I., Nosikov V.V.Med. Sci. Monit. 8:CR180-CR184(2002) A germline single nucleotide polymorphism at the intracellular domain of the human thyrotropin receptor does not have a major effect on the development of Graves' disease.Ban Y., Greenberg D.A., Concepcion E.S., Tomer Y.Thyroid 12:1079-1083(2002) Association of Graves' disease with intragenic polymorphism of the thyrotropin receptor gene in a cohort of Singapore patients of multi-ethnic origins.Ho S.-C., Goh S.-S., Khoo D.H.Thyroid 13:523-528(2003) The human thyrotropin receptor is highly mutable a review of gain-of-function mutations.Farid N.R., Kascur V., Balazs C.Eur. J. Endocrinol. 143:25-30(2000) A somatic point mutation in a putative ligand binding domain of the TSH receptor in a patient with autoimmune hyperthyroidism.Heldin N.-E., Gustavsson B., Westermark K., Westermark B.J. Clin. Endocrinol. Metab. 73:1374-1376(1991) Somatic mutations in the thyrotropin receptor gene cause hyperfunctioning thyroid adenomas.Parma J., Duprez L., van Sande J., Cochaux P., Gervy C., Mockel J., Dumont J.E., Vassart G.Nature 365:649-651(1993) A genomic point mutation in the extracellular domain of the thyrotropin receptor in patients with Graves' ophthalmopathy.Bahn R.S., Dutton C.M., Heufelder A.E., Sarkar G.J. Clin. Endocrinol. Metab. 78:256-260(1994) Novel mutations of thyrotropin receptor gene in thyroid hyperfunctioning adenomas. Rapid identification by fine needle aspiration biopsy.Porcellini A., Ciullo I., Laviola L., Amabile G., Fenzi G., Avvedimento V.E.J. Clin. Endocrinol. Metab. 79:657-661(1994) Identification and functional characterization of two new somatic mutations causing constitutive activation of the thyrotropin receptor in hyperfunctioning autonomous adenomas of the thyroid.Paschke R., Tonacchera M., van Sande J., Parma J., Vassart G.J. Clin. Endocrinol. Metab. 79:1785-1789(1994) Germline mutations in the thyrotropin receptor gene cause non-autoimmune autosomal dominant hyperthyroidism.Duprez L., Parma J., van Sande J., Allgeier A., Leclere J., Schvartz C., Delisle M.-J., Decoulx M., Orgiazzi J., Dumont J.E., Vassart G.Nat. Genet. 7:396-401(1994) Functional analysis of a variant of the thyrotropin receptor gene in a family with Graves' disease.Gustavsson B., Eklof C., Westermark K., Westermark B., Heldin N.-E.Mol. Cell. Endocrinol. 111:167-173(1995) Congenital hyperthyroidism caused by a mutation in the thyrotropin-receptor gene.Kopp P., van Sande J., Parma J., Duprez L., Gerber H., Joss E., Jameson J.L., Dumont J.E., Vassart G.N. Engl. J. Med. 332:150-154(1995) Resistance to thyrotropin caused by mutations in the thyrotropin-receptor gene.Sunthornthepvarakul T., Gottschalk M.E., Hayashi Y., Refetoff S.N. Engl. J. Med. 332:155-160(1995) Point mutations in the thyrotropin receptor in human thyroid tumors.Ohno M., Endo T., Ohta K., Gunji K., Onaya T.Thyroid 5:97-100(1995) Normal function in vivo of a homozygotic polymorphism in the human thyrotropin receptor.Cuddihy R.M., Bryant W.P., Bahn R.S.Thyroid 5:255-257(1995) Functional characteristics of three new germline mutations of the thyrotropin receptor gene causing autosomal dominant toxic thyroid hyperplasia.Tonacchera M., van Sande J., Cetani F., Swillens S., Schvartz C., Winiszewski P., Portmann L., Dumont J.E., Vassart G., Parma J.J. Clin. Endocrinol. Metab. 81:547-554(1996) A neomutation of the thyroid-stimulating hormone receptor in a severe neonatal hyperthyroidism.de Roux N., Polak M., Couet J., Leger J., Czernichow P., Milgrom E., Misrahi M.J. Clin. Endocrinol. Metab. 81:2023-2026(1996) Four families with loss of function mutations of the thyrotropin receptor.de Roux N., Misrahi M., Brauner R., Houang M., Carel J.-C., Granier M., Le Bouc Y., Ghinea N., Boumedienne A., Toublanc J.E., Milgrom E.J. Clin. Endocrinol. Metab. 81:4229-4235(1996) Detection of an activating mutation of the thyrotropin receptor in a case of an autonomously hyperfunctioning thyroid insular carcinoma.Russo D., Tumino S., Arturi F., Vigneri P., Grasso G., Pontecorvi A., Filetti S., Belfiore A.J. Clin. Endocrinol. Metab. 82:735-738(1997) Two novel mutations in the thyrotropin (TSH) receptor gene in a child with resistance to TSH.Clifton-Bligh R.J., Gregory J.W., Ludgate M., John R., Persani L., Asteria C., Beck-Peccoz P., Chatterjee V.K.K.J. Clin. Endocrinol. Metab. 82:1094-1100(1997) Diversity and prevalence of somatic mutations in the thyrotropin receptor and Gs alpha genes as a cause of toxic thyroid adenomas.Parma J., Duprez L., van Sande J., Hermans J., Rocmans P., van Vliet G., Costagliola S., Rodien P., Dumont J.E., Vassart G.J. Clin. Endocrinol. Metab. 82:2695-2701(1997) Mutations of the human thyrotropin receptor gene causing thyroid hypoplasia and persistent congenital hypothyroidism.Biebermann H., Schoeneberg T., Krude H., Schultz G., Gudermann T., Grueters A.J. Clin. Endocrinol. Metab. 82:3471-3480(1997) Sporadic congenital hyperthyroidism due to a spontaneous germline mutation in the thyrotropin receptor gene.Holzapfel H.P., Wonerow P., von Petrykowski W., Henschen M., Scherbaum W.A., Paschke R.J. Clin. Endocrinol. Metab. 82:3879-3884(1997) Identification of a new thyrotropin receptor germline mutation (Leu629Phe) in a family with neonatal onset of autosomal dominant nonautoimmune hyperthyroidism.Fuhrer D., Wonerow P., Willgerodt H., Paschke R.J. Clin. Endocrinol. Metab. 82:4234-4238(1997) Familial congenital hypothyroidism due to inactivating mutation of the thyrotropin receptor causing profound hypoplasia of the thyroid gland.Abramowicz M.J., Duprez L., Parma J., Vassart G., Heinrichs C.J. Clin. Invest. 99:3018-3024(1997) Congenital hyperthyroidism caused by a solitary toxic adenoma harboring a novel somatic mutation (serine281-->isoleucine) in the extracellular domain of the thyrotropin receptor.Kopp P., Muirhead S., Jourdain N., Gu W.X., Jameson J.L., Rodd C.J. Clin. Invest. 100:1634-1639(1997) Congenital nonautoimmune hyperthyroidism in a nonidentical twin caused by a sporadic germline mutation in the thyrotropin receptor gene.Kopp P., Jameson J.L., Roe T.F.Thyroid 7:765-770(1997) Severe congenital hyperthyroidism caused by a germ-line neo mutation in the extracellular portion of the thyrotropin receptor.Grueters A., Schoeneberg T., Biebermann H., Krude H., Krohn H.P., Dralle H., Gudermann T.J. Clin. Endocrinol. Metab. 83:1431-1436(1998) Familial gestational hyperthyroidism caused by a mutant thyrotropin receptor hypersensitive to human chorionic gonadotropin.Rodien P., Bremont C., Raffin Sanson M.-L., Parma J., van Sande J., Costagliola S., Luton J.-P., Vassart G., Duprez L.N. Engl. J. Med. 339:1823-1826(1998) A germline mutation of the thyrotropin receptor gene associated with thyrotoxicosis and mitral valve prolapse in a Chinese family.Khoo D.H.C., Parma J., Rajasoorya C., Ho S.C., Vassart G.J. Clin. Endocrinol. Metab. 84:1459-1462(1999) Germline polymorphism of codon 727 of human thyroid-stimulating hormone receptor is associated with toxic multinodular goiter.Gabriel E.M., Bergert E.R., Grant C.S., van Heerden J.A., Thompson G.B., Morris J.C.J. Clin. Endocrinol. Metab. 84:3328-3335(1999) A Val 677 activating mutation of the thyrotropin receptor in a Hurthle cell thyroid carcinoma associated with thyrotoxicosis.Russo D., Wong M.G., Costante G., Chiefari E., Treseler P.A., Arturi F., Filetti S., Clark O.H.Thyroid 9:13-17(1999) A novel thyrotropin receptor mutation in an infant with severe thyrotoxicosis.Esapa C.T., Duprez L., Ludgate M., Mustafa M.S., Kendall-Taylor P., Vassart G., Harris P.E.Thyroid 9:1005-1010(1999) A novel activating mutation in the thyrotropin receptor gene in an autonomously functioning thyroid nodule developed by a Japanese patient.Kosugi S., Hai N., Okamoto H., Sugawa H., Mori T.Eur. J. Endocrinol. 143:471-477(2000) Analysis of the genetic variability of the 1st (CCC/ACC, P52T) and the 10th exons (bp 1012-1704) of the TSH receptor gene in Graves' disease.Kaczur V., Takacs M., Szalai C., Falus A., Nagy Z., Berencsi G., Balazs C.Eur. J. Immunogenet. 27:17-23(2000) Congenital hypothyroidism with impaired thyroid response to thyrotropin (TSH) and absent circulating thyroglobulin evidence for a new inactivating mutation of the TSH receptor gene.Tonacchera M., Agretti P., Pinchera A., Rosellini V., Perri A., Collecchi P., Vitti P., Chiovato L.J. Clin. Endocrinol. Metab. 85:1001-1008(2000) Activating thyrotropin receptor mutations are present in nonadenomatous hyperfunctioning nodules of toxic or autonomous multinodular goiter.Tonacchera M., Agretti P., Chiovato L., Rosellini V., Ceccarini G., Perri A., Viacava P., Naccarato A.G., Miccoli P., Pinchera A., Vitti P.J. Clin. Endocrinol. Metab. 85:2270-2274(2000) Lack of association of nonautoimmune hyperfunctioning thyroid disorders and a germline polymorphism of codon 727 of the human thyrotropin receptor in a European Caucasian population.Muehlberg T., Herrmann K., Joba W., Kirchberger M., Heberling H.-J., Heufelder A.E.J. Clin. Endocrinol. Metab. 85:2640-2643(2000) A novel mutation in the thyrotropin (TSH) receptor gene causing loss of TSH binding but constitutive receptor activation in a family with resistance to TSH.Russo D., Betterle C., Arturi F., Chiefari E., Girelli M.E., Filetti S.J. Clin. Endocrinol. Metab. 85:4238-4242(2000) Constitutively activating TSH-receptor mutations as a molecular cause of non-autoimmune hyperthyroidism in childhood.Biebermann H., Schoeneberg T., Krude H., Gudermann T., Grueters A.Langenbecks Arch. Surg. 385:390-392(2000) Sporadic nonautoimmune congenital hyperthyroidism due to a strong activating mutation of the thyrotropin receptor gene.Tonacchera M., Agretti P., Rosellini V., Ceccarini G., Perri A., Zampolli M., Longhi R., Larizza D., Pinchera A., Vitti P., Chiovato L.Thyroid 10:859-863(2000) A Phe 486 thyrotropin receptor mutation in an autonomously functioning follicular carcinoma that was causing hyperthyroidism.Camacho P., Gordon D., Chiefari E., Yong S., DeJong S., Pitale S., Russo D., Filetti S.Thyroid 10:1009-1012(2000) Novel TSHR germline mutation (Met463Val) masquerading as Graves' disease in a large Welsh kindred with hyperthyroidism.Fuhrer D., Warner J., Sequeira M., Paschke R., Gregory J.W., Ludgate M.Thyroid 10:1035-1041(2000) A novel germline mutation in the TSH receptor gene causes non-autoimmune autosomal dominant hyperthyroidism.Alberti L., Proverbio M.C., Costagliola S., Weber G., Beck-Peccoz P., Chiumello G., Persani L.Eur. J. Endocrinol. 145:249-254(2001) The first activating TSH receptor mutation in transmembrane domain 1 identified in a family with nonautoimmune hyperthyroidism.Biebermann H., Schoeneberg T., Hess C., Germak J., Gudermann T., Grueters A.J. Clin. Endocrinol. Metab. 86:4429-4433(2001) Detection of thyroid-stimulating hormone receptor and G(s) alpha mutations in 75 toxic thyroid nodules by denaturing gradient gel electrophoresis.Truelzsch B., Krohn K., Wonerow P., Chey S., Holzapfel H.-P., Ackermann F., Fuehrer D., Paschke R.J. Mol. Med. 78:684-691(2001) Novel inactivating missense mutations in the thyrotropin receptor gene in Japanese children with resistance to thyrotropin.Nagashima T., Murakami M., Onigata K., Morimura T., Nagashima K., Mori M., Morikawa A.Thyroid 11:551-559(2001) Oncogenic mutations in the thyrotropin receptor of autonomously functioning thyroid nodules in the Japanese population.Vanvooren V., Uchino S., Duprez L., Costa M.J., Vandekerckhove J., Parma J., Vassart G., Dumont J.E., van Sande J., Noguchi S.Eur. J. Endocrinol. 147:287-291(2002) Germline mutations of TSH receptor gene as cause of nonautoimmune subclinical hypothyroidism.Alberti L., Proverbio M.C., Costagliola S., Romoli R., Boldrighini B., Vigone M.C., Weber G., Chiumello G., Beck-Peccoz P., Persani L.J. Clin. Endocrinol. Metab. 87:2549-2555(2002) Functional significance of the thyrotropin receptor germline polymorphism D727E.Sykiotis G.P., Neumann S., Georgopoulos N.A., Sgourou A., Papachatzopoulou A., Markou K.B., Kyriazopoulou V., Paschke R., Vagenakis A.G., Papavassiliou A.G.Biochem. Biophys. Res. Commun. 301:1051-1056(2003) Polymorphisms in thyroid hormone pathway genes are associated with plasma TSH and iodothyronine levels in healthy subjects.Peeters R.P., van Toor H., Klootwijk W., de Rijke Y.B., Kuiper G.G.J.M., Uitterlinden A.G., Visser T.J.J. Clin. Endocrinol. Metab. 88:2880-2888(2003) TSH receptor and Gs(alpha) genetic analysis in children with Down's syndrome and subclinical hypothyroidism.Tonacchera M., Perri A., De Marco G., Agretti P., Montanelli L., Banco M.E., Corrias A., Bellone J., Tosi M.T., Vitti P., Martino E., Pinchera A., Chiovato L.J. Endocrinol. Invest. 26:997-1000(2003) Congenital hypothyroidism and apparent athyreosis with compound heterozygosity or compensated hypothyroidism with probable hemizygosity for inactivating mutations of the TSH receptor.Park S.-M., Clifton-Bligh R.J., Betts P., Chatterjee V.K.K.Clin. Endocrinol. (Oxf.) 60:220-227(2004) Premature birth and low birth weight associated with nonautoimmune hyperthyroidism due to an activating thyrotropin receptor gene mutation.Vaidya B., Campbell V., Tripp J.H., Spyer G., Hattersley A.T., Ellard S.Clin. Endocrinol. (Oxf.) 60:711-718(2004) Low prevalence of thyrotropin receptor mutations in a large series of subjects with sporadic and familial nonautoimmune subclinical hypothyroidism.Tonacchera M., Perri A., De Marco G., Agretti P., Banco M.E., Di Cosmo C., Grasso L., Vitti P., Chiovato L., Pinchera A.J. Clin. Endocrinol. Metab. 89:5787-5793(2004) +Additional computationally mapped references.<p>Provides general information on the entry.

NCBI and Uniprot Product Information

• NCBI GI #: 64085121
• NCBI GeneID: 7253
• NCBI Accession #: NP_000360.2
• NCBI GenBank Nucleotide #: NM_000369.2
• UniProt Accession #: P16473; Q16503; Q8TB90; Q96GT6; Q9P1V4; Q9ULA3; Q9UPH3; A0PJU7; F5GYU5; G3V2A9
• NCBI Official Full Name: thyrotropin receptor isoform 1
• NCBI Official Synonym Full Names: thyroid stimulating hormone receptor
• NCBI Official Symbol: TSHR
• NCBI Official Synonym Symbols: LGR3; CHNG1; hTSHR-I
• NCBI Protein Information: thyrotropin receptor
• UniProt Protein Name: Thyrotropin receptor
• UniProt Gene Name: TSHR
• UniProt Synonym Gene Names: LGR3; TSH-R
• UniProt Entry Name: TSHR_HUMAN