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Standard Curve (Sample)

Mouse Tumour necrosis factor related weak inducer of apoptosis ELISA Kit | TWEAK elisa kit

Mouse Tumour necrosis factor related weak inducer of apoptosis ELISA Kit

Reactivity
Mouse
Synonyms
Tumour necrosis factor related weak inducer of apoptosis; N/A; Mouse Tumour necrosis factor related weak inducer of apoptosis ELISA Kit; TWEAK elisa kit
Ordering
 
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For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of TNF-RWIA. No significant cross-reactivity or interference between TNF-RWIA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TNF-RWIA and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for TWEAK elisa kit
Principle of the Assay: TNF-RWIA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TNF-RWIA. Standards or samples are then added to the microtiter plate wells and TNF-RWIA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TNF-RWIA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TNF-RWIA are added to each well to "sandwich" the TNF-RWIA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNF-RWIA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNF-RWIA concentration in each sample is interpolated from this standard curve.

Intended Uses: This TNF-RWIA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TNF-RWIA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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Product Notes

The Mouse TWEAK (Catalog #AAA16062) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16062 ELISA Kit recognizes Mouse TWEAK. It is sometimes possible for the material contained within the vial of "Tumour necrosis factor related weak inducer of apoptosis, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.