Human Thioredoxin ELISA Kit | TXN elisa kit

Human Thioredoxin PicoKine ELISA Kit

Cat. Num. AAA45986

• Gene Names: TXN; TRX; TRDX; TRX1
• Reactivity: Human
• Synonyms: Thioredoxin; N/A; Human Thioredoxin PicoKine ELISA Kit; thioredoxin; ADF; ATL-derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; THIO_HUMAN; TRDX; TRX 1; Trx; TRX1; TXN; TXN protein; TXN elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Thioredoxin
• Sequence Length: 105
• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human Thioredoxin PicoKine ELISA Kit standard curve)

Related Product Information for TXN elisa kit

Principle of the assay: human Thioredoxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Thioredoxin has been precoated onto 96-well plates. Standards(E.coli, V2-V105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thioredoxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Thioredoxin amount of sample captured in plate.
Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 135773
• NCBI GeneID: 7295
• UniProt Accession #: P10599; O60744; Q53X69; Q96KI3; Q9UDG5; B1ALW1
• Molecular Weight: 9,452 Da
• NCBI Official Full Name: Thioredoxin
• NCBI Official Synonym Full Names: thioredoxin
• NCBI Official Symbol: TXN
• NCBI Official Synonym Symbols: TRX; TRDX; TRX1
• NCBI Protein Information: thioredoxin; ADF; SASP; TXN delta 3; ATL-derived factor; thioredoxin delta 3; surface-associated sulphydryl protein
• UniProt Protein Name: Thioredoxin
• UniProt Gene Name: TXN
• UniProt Synonym Gene Names: TRDX; TRX; TRX1; Trx; ADF; SASP
• UniProt Entry Name: THIO_HUMAN