Mouse TYRO protein tyrosine kinase-binding protein ELISA Kit | Tyrobp elisa kit

Mouse TYRO protein tyrosine kinase-binding protein ELISA Kit

Cat. Num. AAA191541

• Gene Names: Tyrobp; Ly83; DAP12; KARAP
• Reactivity: Mouse
• Synonyms: TYRO protein tyrosine kinase-binding protein; N/A; Mouse TYRO protein tyrosine kinase-binding protein ELISA Kit; DNAX-activation protein 12; Killer-activating receptor-associated protein; KAR-associated protein; Tyrobp; Dap12; Karap; Tyrobp elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse TYRO protein tyrosine kinase-binding protein
• Sequence Length: 114
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: < 0.163 ng/mL

• Intra-assay Precision: <=4.9%
• Inter-assay Precision: <=7.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Standard Curve (Sample)

Related Product Information for Tyrobp elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 6755915
• NCBI GeneID: 22177
• NCBI Accession #: NP_035792.1
• NCBI GenBank Nucleotide #: NM_011662.2
• UniProt Accession #: O54885; O88603
• Molecular Weight: 12,367 Da
• NCBI Official Full Name: TYRO protein tyrosine kinase-binding protein
• NCBI Official Synonym Full Names: TYRO protein tyrosine kinase binding protein
• NCBI Official Symbol: Tyrobp
• NCBI Official Synonym Symbols: Ly83; DAP12; KARAP
• NCBI Protein Information: TYRO protein tyrosine kinase-binding protein
• UniProt Protein Name: TYRO protein tyrosine kinase-binding protein
• UniProt Gene Name: Tyrobp
• UniProt Synonym Gene Names: Dap12; Karap; KAR-associated protein
• UniProt Entry Name: TYOBP_MOUSE