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product-image-AAA90644_SC15.jpg Standard Curve (Sample)

Porcine Urokinase-type Plasminogen Activator Receptor ELISA Kit | u-PAR elisa kit

Porcine Urokinase-type Plasminogen Activator Receptor ELISA Kit

Gene Names
PLAUR; CD87; UPAR; URKR; U-PAR
Reactivity
Porcine
Synonyms
Urokinase-type Plasminogen Activator Receptor; N/A; Porcine Urokinase-type Plasminogen Activator Receptor ELISA Kit; u-PAR elisa kit
Ordering
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This uPAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine uPAR. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

product-image-AAA90644_SC15.jpg Standard Curve (Sample)
Related Product Information for u-PAR elisa kit
Principle of the Assay: uPAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-uPAR antibody and an uPAR-HRP conjugate. The assay sample and buffer are incubated together with uPAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the uPAR concentration since uPAR from samples and uPAR-HRP conjugate compete for the anti-uPAR antibody binding site. Since the number of sites is limited, as more sites are occupied by uPAR from the sample, fewer sites are left to bind uPAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The uPAR concentration in each sample is interpolated from this standard curve.
Product Categories/Family for u-PAR elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
32,016 Da
NCBI Official Full Name
urokinase-type plasminogen activator receptor
NCBI Official Synonym Full Names
plasminogen activator, urokinase receptor
NCBI Official Symbol
PLAUR
NCBI Official Synonym Symbols
CD87; UPAR; URKR; U-PAR
NCBI Protein Information
urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; urokinase-type plasminogen activator (uPA) receptor
UniProt Protein Name
Urokinase plasminogen activator surface receptor
UniProt Gene Name
PLAUR
UniProt Synonym Gene Names
MO3; UPAR; U-PAR; uPAR
UniProt Entry Name
UPAR_HUMAN

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Product Notes

The Porcine u-PAR plaur (Catalog #AAA90644) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA90644 ELISA Kit recognizes Porcine u-PAR. It is sometimes possible for the material contained within the vial of "Urokinase-type Plasminogen Activator Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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