Ubiquitin Activating Enzyme E1/UBA1 Recombinant Protein | UBA1 recombinant protein

Ubiquitin Activating Enzyme E1/UBA1 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA258001

• Gene Names: UBA1; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Ubiquitin Activating Enzyme E1/UBA1; N/A; Ubiquitin Activating Enzyme E1/UBA1 Protein, Human, Recombinant (His & GST Tag); Human UBE1/UBA1 Protein (His & GST Tag); A1S9 Protein, Human; A1S9T Protein, Human; A1ST Protein, Human; AMCX1 Protein, Human; CFAP124 Protein, Human; CTD-2522E6.1 Protein, Human; GXP1 Protein, Human; POC20 Protein, Human; SMAX2 Protein, Human; UBA1A Protein, Human; UBE1 Protein, Human; UBE1X Protein, Human; ubiquitin-like modifier activating enzyme 1; UBA1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 10% gly, 0.5mM GSH.; Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence: Ser2-Arg1058

• Source: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: GST & His
• Protein Construction: A DNA sequence encoding the human UBA1 (NP_003325.2)(Ser 2-Arg 1058) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: It is recommended that sterile water (200ul) be added to the vial to prepare a stock solution of 0.25mg/mL.; Concentration is measured BCA Method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Related Product Information for UBA1 recombinant protein

Background: UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Defects in UBA1 can cause spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.

References

Jin J, et al. (2007) Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature. 447(7148):1135-8.Xia T, et al. (2007) Chaperone-dependent E3 ligase CHIP ubiquitinates and mediates proteasomal degradation of soluble guanylyl cyclase. Am J Physiol Heart Circ Physiol. 293(5):H3080-7.Pridgeon JW, et al. (2009) Proteomic analysis reveals Hrs UIM-mediated ubiquitin signaling in multiple cellular processes. FEBS J. 276(1):118-31.

NCBI and Uniprot Product Information

• NCBI GI #: 23510338
• NCBI GeneID: 7317
• NCBI Accession #: NP_003325.2
• NCBI GenBank Nucleotide #: NM_003334.3
• UniProt Accession #: P22314; Q5JRR8; Q96E13
• Molecular Weight: Molecular Mass: The recombinant human UBA1/GST chimera consists of 1294 amino acids and has a calculated molecular mass of 146 KDa. It migrates as an approximately 130 KDa band in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 1
• NCBI Official Synonym Full Names: ubiquitin like modifier activating enzyme 1
• NCBI Official Symbol: UBA1
• NCBI Official Synonym Symbols: A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 1
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 1
• UniProt Gene Name: UBA1
• UniProt Synonym Gene Names: A1S9T; UBE1
• UniProt Entry Name: UBA1_HUMAN