Application Data
(AMP-Glo assay for UBA1 activity 7.9uM ubiquitin and 25uM ATP were incubated with different concentrations of UBA1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBA1 recombinant protein
Recombinant UBA1 protein
Purity
>=95% by SDS-PAGE.
Synonyms
UBA1; N/A; Recombinant UBA1 protein; Ubiquitin-like modifier-activating; A1S9; A1S9T; A1ST; GXP1; UBE1; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124; UBA1 recombinant protein
Host
Baculovirus
Purity/Purification
>=95% by SDS-PAGE.
Form/Format
Recombinant UBA1 protein is supplied in 25 mM HEPES pH 7.5, 500 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant UBA1 protein that includes full length of human UBA1 protein (accession number NP_003325.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with a molecular weight of 119.12kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(AMP-Glo assay for UBA1 activity 7.9uM ubiquitin and 25uM ATP were incubated with different concentrations of UBA1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
Application Data
(AMP-Glo assay for UBA1 activity 7.9uM ubiquitin and 25uM ATP were incubated with different concentrations of UBA1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE
(Recombinant UBA1 protein gel. UBA1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 119.12kDa Purity: >95%)
SDS-PAGE
(Recombinant UBA1 protein gel. UBA1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 119.12kDa Purity: >95%)
Related Product Information for UBA1 recombinant protein
UBA1 (Ubiquitin-like modifier-activating) also known as A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X and VEXAS. UBA1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
Product Categories/Family for UBA1 recombinant protein
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Product Notes
The UBA1 (Catalog #AAA60404) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "UBA1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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