Application Data
(AMP-Glo assay for UBE2L3 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2L3 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2L3 recombinant protein
Recombinant UBE2L3 protein
Purity
>=95% by SDS-PAGE.
Synonyms
UBE2L3; N/A; Recombinant UBE2L3 protein; Ubiqutin conjugating enzyme E2 L3; E2-F1; L-UBC; UBCH7; UbcM4; UBCE7; UBE2L3 recombinant protein
Host
E coli
Purity/Purification
>=95% by SDS-PAGE.
Form/Format
Recombinant UBE2L3 protein is supplied in 25 mM Tris 8.0, 300mM NaCl, 20% glycerol, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant UBE2L3 protein that includes full length of human UBE2L3 protein (accession number NP_003338.1) was expressed in E coli and contains an N-terminal His tag with a molecular weight of 20.02kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(AMP-Glo assay for UBE2L3 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2L3 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
Application Data
(AMP-Glo assay for UBE2L3 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2L3 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE
(Recombinant UBEL3 protein gel. 12.5% SDS-PAGE Coomassie staining MW: 20.02kDa Purity: ?95%)
SDS-PAGE
(Recombinant UBEL3 protein gel. 12.5% SDS-PAGE Coomassie staining MW: 20.02kDa Purity: ?95%)
Related Product Information for UBE2L3 recombinant protein
UBE2L3 (Ubiquitin-conjugating enzyme E2 L3) also known as E2-F1, L-UBC, UBCH7 and UbcM4. UBE2L3 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. It has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes ‘Lys-11’-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
Product Categories/Family for UBE2L3 recombinant protein
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Product Notes
The UBE2L3 (Catalog #AAA60392) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "UBE2L3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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