SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
USP15 recombinant protein
USP15, Active
Applications
Western Blot
Synonyms
USP15; N/A; USP15, Active; KIAA0529; UNPH-2; USP15 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Applicable Applications for USP15 recombinant protein
WB (Western Blot), Activity Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for USP15 recombinant protein
Recombinant full-length human USP15 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The USP15 gene accession number is NM_001252078.
USP15 (ubiquitin carboxyl-terminal hydrolase 15) is a member of the ubiquitin specific protease (USP) family of cysteine protease deubiquitination enzymes and regulates TGFB receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways. The enzyme interacts with OP9 signalosome and is a key regulator of TGFB receptor signaling through deubiquitination of receptor-activated SMAD transcription factors (1). USP15 also plays a role in the regulation and stabilization of Human Papillomavirus (HPV) type 16 E6 protein, suggesting that E6 may be a target for USP15 DUB activity (2).
USP15 (ubiquitin carboxyl-terminal hydrolase 15) is a member of the ubiquitin specific protease (USP) family of cysteine protease deubiquitination enzymes and regulates TGFB receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways. The enzyme interacts with OP9 signalosome and is a key regulator of TGFB receptor signaling through deubiquitination of receptor-activated SMAD transcription factors (1). USP15 also plays a role in the regulation and stabilization of Human Papillomavirus (HPV) type 16 E6 protein, suggesting that E6 may be a target for USP15 DUB activity (2).
Product Categories/Family for USP15 recombinant protein
References
1. Inui, M. et al: USP15 is a deubiquitylating enzyme for receptor-activated SMADs. Nat Cell Biol. 13(11):1368-75, 2011
2. Vos, Robin M et al: The ubiquitin-specific peptidase USP15 regulates human papillomavirus type 16 E6 protein stability. Journal of virology 83(17): 8885-92, 2009.
2. Vos, Robin M et al: The ubiquitin-specific peptidase USP15 regulates human papillomavirus type 16 E6 protein stability. Journal of virology 83(17): 8885-92, 2009.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The USP15 (Catalog #AAA74102) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's USP15 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Activity Assay. Researchers should empirically determine the suitability of the USP15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP15, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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