Standard Curve (Sample)
Human VCAM-1 ELISA Kit | VCAM1 elisa kit
Human VCAM-1 PicoKine ELISA Kit
Gene Names
VCAM1; CD106; INCAM-100
Reactivity
Human
Synonyms
VCAM-1; N/A; Human VCAM-1 PicoKine ELISA Kit; Vascular cell adhesion protein 1; vascular cell adhesion molecule 1; CD106; CD106 antigen; DKFZp779G2333; INCAM 100; INCAM-100; INCAM100; L1CAM; MGC99561; V CAM 1; V-CAM 1; Vascular cell adhesion molecule 1; VCAM 1; VCAM1; VCAM1_HUMAN; VCAM1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human VCAM1
Sequence Length
739
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for VCAM1 elisa kit
Background: Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.
Principle of the Assay: The Picokine™ Human VCAM1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human VCAM1 with a 96-well strip plate that is pre-coated with antibody specific for VCAM1. The detection antibody is a biotinylated antibody specific for VCAM1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human VCAM1 protein as standards. To measure Human VCAM1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human VCAM1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human VCAM1 in the sample.
Principle of the Assay: The Picokine™ Human VCAM1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human VCAM1 with a 96-well strip plate that is pre-coated with antibody specific for VCAM1. The detection antibody is a biotinylated antibody specific for VCAM1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human VCAM1 protein as standards. To measure Human VCAM1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human VCAM1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human VCAM1 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
74,346 Da
NCBI Official Full Name
Vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
VCAM1
NCBI Official Synonym Symbols
CD106; INCAM-100
NCBI Protein Information
vascular cell adhesion protein 1; CD106 antigen
UniProt Protein Name
Vascular cell adhesion protein 1
UniProt Gene Name
VCAM1
UniProt Synonym Gene Names
L1CAM; V-CAM 1; VCAM-1
UniProt Entry Name
VCAM1_HUMAN
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Product Notes
The Human VCAM1 vcam1 (Catalog #AAA11576) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11576 ELISA Kit recognizes Human VCAM1. It is sometimes possible for the material contained within the vial of "VCAM-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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