Human vinculin ELISA Kit | VCL elisa kit

Human Vinculin, VCL ELISA Kit

Cat. Num. AAA112404

• Gene Names: VCL; MV; MVCL; CMD1W; CMH15; HEL114
• Reactivity: Human
• Synonyms: vinculin; N/A; Human Vinculin, VCL ELISA Kit; Human Vinculin (VCL) ELISA kit; RP11-178G16.3; CMD1W; MVCL; metavinculin; VCL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human VCL. No significant cross-reactivity or interference between human VCL and analogues was observed.
• Sequence Length: 1066
• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 28 pg/ml -1800 pg/ml.
• Sensitivity: The minimum detectable dose of human VCL is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for VCL elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VCL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4507877
• NCBI GeneID: 7414
• NCBI Accession #: NP_003364.1
• NCBI GenBank Nucleotide #: NM_003373.3
• UniProt Accession #: P18206; Q16450; Q5SWX2; Q7Z3B8; Q8IXU7
• Molecular Weight: 123,799 Da
• NCBI Official Full Name: vinculin isoform VCL
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: VCL
• NCBI Official Synonym Symbols: MV; MVCL; CMD1W; CMH15; HEL114
• NCBI Protein Information: vinculin; metavinculin; epididymis luminal protein 114
• UniProt Protein Name: Vinculin
• UniProt Gene Name: VCL
• UniProt Synonym Gene Names: MV
• UniProt Entry Name: VINC_HUMAN