Human vascular endothelial growth factor C ELISA Kit | VEGFC elisa kit

Human Vascular Endothelial cell Growth Factor C, VEGF-C ELISA Kit

Cat. Num. AAA80746

• Gene Names: VEGFC; VRP; Flt4-L; LMPH1D
• Reactivity: Human
• Synonyms: vascular endothelial growth factor C; N/A; Human Vascular Endothelial cell Growth Factor C, VEGF-C ELISA Kit; Human Vascular Endothelial cell Growth Factor C; VEGF-C ELISA KIT; Flt4-L; VRP; FLT4 ligand DHM; vascular endothelial growth factor-related protein; VEGFC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human VEGFC. No significant cross-reactivity or interference between Human VEGFC and analogues was observed.
• Sequence Length: 419
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 3.9 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Angiogenesis
• Protein Biological Process 3: Angiogenesis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for VEGFC elisa kit

Introduction: Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D).

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for VEGF-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain VEGF-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of VEGF-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.4
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 46,883 Da
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L; LMPH1D
• NCBI Protein Information: vascular endothelial growth factor C; FLT4 ligand DHM; vascular endothelial growth factor-related protein
• UniProt Protein Name: Vascular endothelial growth factor C
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN