SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
VPS4B recombinant protein
VPS4B, 1-444aa, Human, His tag, E Coli
Gene Names
VPS4B; MIG1; SKD1; SKD1B; VPS4-2
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
VPS4B; N/A; VPS4B, 1-444aa, Human, His tag, E Coli; Vacuolar protein sorting-associated protein 4B; MIG1; SKD1; SKD1B; VPS4-2; VPS4B recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Concentration
0.25mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYL DRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSEN ESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVN EHDLLKLKKF TEDFGQEG
Sequence Length
444
Applicable Applications for VPS4B recombinant protein
SDS-PAGE
Tag Information
His-tag
Preparation and Storage
Store at 2-8°C for a week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for VPS4B recombinant protein
Vacuolar protein sorting-associated protein 4B, also known as VPS4B, belongs to the AAA (ATPases associated with diverse cellular activities) family. It is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Dominant negative mutant of VPS4B inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding. Recombinant human VPS4B protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for VPS4B recombinant protein
References
Scheuring S., et al. (2001) J Mol Biol. 312:469-480.; Perez O D., et al. (2001) Immunity. 15:687-690
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51.8 kDa (468aa)
NCBI Official Full Name
vacuolar protein sorting-associated protein 4B
NCBI Official Synonym Full Names
vacuolar protein sorting 4 homolog B
NCBI Official Symbol
VPS4B
NCBI Official Synonym Symbols
MIG1; SKD1; SKD1B; VPS4-2
NCBI Protein Information
vacuolar protein sorting-associated protein 4B
UniProt Protein Name
Vacuolar protein sorting-associated protein 4B
UniProt Gene Name
VPS4B
UniProt Synonym Gene Names
SKD1; VPS42; Protein SKD1
Similar Products
Product Notes
The VPS4B vps4b (Catalog #AAA48855) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's VPS4B can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the VPS4B vps4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYL DRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSEN ESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVN EHDLLKLKKF TEDFGQEG. It is sometimes possible for the material contained within the vial of "VPS4B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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