Monkey Von Willebrand Factor ELISA Kit | Vwf elisa kit

Monkey Von Willebrand Factor ELISA Kit

Cat. Num. AAA88914

• Gene Names: Vwf; VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik
• Reactivity: Monkey
• Synonyms: Von Willebrand Factor; N/A; Monkey Von Willebrand Factor ELISA Kit; Vwf elisa kit

• Reactivity: Monkey
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000ng/mL
• Sensitivity: 1.0ng/mL

• Intended Uses: This VWF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey VWF. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Vwf elisa kit

Principle of the Assay: VWF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VWF. Standards or samples are then added to the microtiter plate wells and VWF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VWF present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for VWF are added to each well to "sandwich" the VWF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VWF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VWF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Vwf elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 86129842
• NCBI GeneID: 22371
• Molecular Weight: 44,467 Da
• NCBI Official Full Name: VWF
• NCBI Official Synonym Full Names: Von Willebrand factor homolog
• NCBI Official Symbol: Vwf
• NCBI Official Synonym Symbols: VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik
• NCBI Protein Information: von Willebrand factor
• UniProt Protein Name: von Willebrand factor
• UniProt Gene Name: Vwf
• UniProt Synonym Gene Names: vWF
• UniProt Entry Name: VWF_MOUSE