WB (Western Blot)
(Recombinant NSD3 (WHSC1L1)-SET activity assay. 2 ug recombinant nucleosomes were incubated with NSD3 (WHSC1L1)-SET protein in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at RT. 5 ul reaction products were loaded on a 12.5% SDS-PAGE gel. Western Blot was used to detect reaction products (H3K36me2). NSD3 (WHSC1L1)-SET only was used as negative control. Results show that the activity of NSD3 (WHSC1L1)-SET with nucleosomes as substrate is much better than its activity with histone octamers as substrate.)
NSD3 recombinant protein
Recombinant NSD3 (WHSC1L1)-SET protein
Gene Names
NSD3; KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
Synonyms
NSD3; N/A; Recombinant NSD3 (WHSC1L1)-SET protein; pp14328; NSD3 recombinant protein
Host
E Coli
Form/Format
Recombinant NSD3 (WHSC1L1)-SET (a.a. 1021-1320) is supplied in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant NSD3 (WHSC1L1)-SET protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 2ug recombinant nucleosomes were incubated with different concentrations of recombinant NSD3 (WHSC1L1)-SET protein in 30ul reaction system containing 50mM TrisCl, pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 50uM SAM for 3 hours at room temperature. Activity was detected by Western Blot.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
WB (Western Blot)
(Recombinant NSD3 (WHSC1L1)-SET activity assay. 2 ug recombinant nucleosomes were incubated with NSD3 (WHSC1L1)-SET protein in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at RT. 5 ul reaction products were loaded on a 12.5% SDS-PAGE gel. Western Blot was used to detect reaction products (H3K36me2). NSD3 (WHSC1L1)-SET only was used as negative control. Results show that the activity of NSD3 (WHSC1L1)-SET with nucleosomes as substrate is much better than its activity with histone octamers as substrate.)
WB (Western Blot)
(Recombinant NSD3 (WHSC1L1)-SET activity assay. 2 ug recombinant nucleosomes were incubated with NSD3 (WHSC1L1)-SET protein in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at RT. 5 ul reaction products were loaded on a 12.5% SDS-PAGE gel. Western Blot was used to detect reaction products (H3K36me2). NSD3 (WHSC1L1)-SET only was used as negative control. Results show that the activity of NSD3 (WHSC1L1)-SET with nucleosomes as substrate is much better than its activity with histone octamers as substrate.)
SDS-PAGE
(Recombinant NSD3 (WHSC1L1)-SET protein gel. Recombinant NSD3 (WHSC1L1)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 60kDa Purity: > 90%)
SDS-PAGE
(Recombinant NSD3 (WHSC1L1)-SET protein gel. Recombinant NSD3 (WHSC1L1)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 60kDa Purity: > 90%)
Related Product Information for NSD3 recombinant protein
Short Description: Recombinant NSD3 (WHSC1L1)-SET peptide that includes amino acids 1021-1320 that contains the SET domain sequence of the human NSD3 (WHSC1L1) protein (accession number NP_075447.1) was expressed in E Coli and contains an N-terminal GST-Tag with an observed molecular weight of 60 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: NSD3 (WHSC1L1/Wolf-Hirschhorn Syndrome Candidtae 1-Like 1) is a histone methyltransferase of NSD (nuclear receptor binding SET domain protein) family. NSD3 (WHSC1L1) can recognize and methylate several lysine marks if using histone as substrate. However, if using nucleosome as substrate, NSD3 (WHSC1L1) preferentially methylates lysine 36 of histone H3. The NSD3 (WHSC1L1) gene is amplified in several cancers, including lung cancer and head and neck cancer, and may play a role in carcinogenesis. NSD3 (WHSC1L1) includes the SET domain of NSD3 (WHSC1L1). The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in Drosophila proteins Su(var)3-9, EZH2, and TrxG, and plays a crucial role in substrate recognition and enzymatic activity.
Background: NSD3 (WHSC1L1/Wolf-Hirschhorn Syndrome Candidtae 1-Like 1) is a histone methyltransferase of NSD (nuclear receptor binding SET domain protein) family. NSD3 (WHSC1L1) can recognize and methylate several lysine marks if using histone as substrate. However, if using nucleosome as substrate, NSD3 (WHSC1L1) preferentially methylates lysine 36 of histone H3. The NSD3 (WHSC1L1) gene is amplified in several cancers, including lung cancer and head and neck cancer, and may play a role in carcinogenesis. NSD3 (WHSC1L1) includes the SET domain of NSD3 (WHSC1L1). The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in Drosophila proteins Su(var)3-9, EZH2, and TrxG, and plays a crucial role in substrate recognition and enzymatic activity.
Product Categories/Family for NSD3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,314 Da
NCBI Official Full Name
histone-lysine N-methyltransferase NSD3 isoform long
NCBI Official Synonym Full Names
nuclear receptor binding SET domain protein 3
NCBI Official Symbol
NSD3
NCBI Official Synonym Symbols
KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
NCBI Protein Information
histone-lysine N-methyltransferase NSD3
UniProt Protein Name
Histone-lysine N-methyltransferase NSD3
UniProt Gene Name
WHSC1L1
UniProt Synonym Gene Names
NSD3; WHSC1-like protein 1
UniProt Entry Name
NSD3_HUMAN
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Product Notes
The NSD3 whsc1l1 (Catalog #AAA59707) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NSD3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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