SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
WIP1 recombinant protein
WIP1, Active
Gene Names
PPM1D; WIP1; PP2C-DELTA
Applications
Western Blot
Synonyms
WIP1; N/A; WIP1, Active; PPM1D; JDVS; IDDGIP; PP2C-DELTA; WIP1 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 20mM MOPS, pH7.5, 300mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for WIP1 recombinant protein
WB (Western Blot), Phosphatase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for WIP1 recombinant protein
Recombinant full-length human WIP1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC016480.
Wild-type p53-induced phosphatase 1 (WIP1) is a member of the PP2C family of Ser/Thr protein, and is highly expressed in testis and ovary (1). The expression of WIP1 is induced in responses to the environmental stresses (2). WIP1 dephosphorylates CHK2 at Thr-68 in as a negative regulator in the response of DNA damage (2). WIP1 suppresses the DNA double strand break repair by dephosphorylating the phosphorylated Ser-139 in a histone variant gamma-H2AX (3).
Wild-type p53-induced phosphatase 1 (WIP1) is a member of the PP2C family of Ser/Thr protein, and is highly expressed in testis and ovary (1). The expression of WIP1 is induced in responses to the environmental stresses (2). WIP1 dephosphorylates CHK2 at Thr-68 in as a negative regulator in the response of DNA damage (2). WIP1 suppresses the DNA double strand break repair by dephosphorylating the phosphorylated Ser-139 in a histone variant gamma-H2AX (3).
Product Categories/Family for WIP1 recombinant protein
References
1. Fiscella M. et. al: Wip1, a novel human protein phosphatase that is induced in response to ionizing radiation in a p53-dependent manner. PNAS. 1997 Jun 10; 94(12):6048-53.
2. Yoda A. et. al: Intrinsic kinase activity and SQ/TQ domain of Chk2 kinase as well as N-terminal domain of Wip1 phosphatase are required for regulation of Chk2 by Wip1. J Biol Chem. 2006 Aug 25; 281(34):24847-62.
3. Moon S. H. et. al: Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. J Biol Chem. 2010 Apr 23; 285(17):12935-47.
2. Yoda A. et. al: Intrinsic kinase activity and SQ/TQ domain of Chk2 kinase as well as N-terminal domain of Wip1 phosphatase are required for regulation of Chk2 by Wip1. J Biol Chem. 2006 Aug 25; 281(34):24847-62.
3. Moon S. H. et. al: Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. J Biol Chem. 2010 Apr 23; 285(17):12935-47.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47,211 Da
NCBI Official Full Name
Homo sapiens protein phosphatase 1D magnesium-dependent, delta isoform, mRNA
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1D
NCBI Official Symbol
PPM1D
NCBI Official Synonym Symbols
WIP1; PP2C-DELTA
NCBI Protein Information
protein phosphatase 1D
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Product Notes
The WIP1 (Catalog #AAA73786) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's WIP1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Phosphatase Assay. Researchers should empirically determine the suitability of the WIP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WIP1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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