Standard Curve (Sample)
Human wingless-type MMTV integration site family, member 4 ELISA Kit | WNT4 elisa kit
Human Protein Wnt-4, WNT4 ELISA Kit
Gene Names
WNT4; WNT-4; SERKAL
Reactivity
Human
Synonyms
wingless-type MMTV integration site family, member 4; N/A; Human Protein Wnt-4, WNT4 ELISA Kit; Human Protein Wnt-4 (WNT4) ELISA kit; RP1-224A6.7; SERKAL; WNT-4; WNT-4 protein; wingless-type MMTV integration site family; member 4; WNT4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human WNT4. No significant cross-reactivity or interference between human WNT4 and analogues was observed.
Sequence Length
351
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
23.5 pg/ml -1500 pg/ml.
Sensitivity
The minimum detectable dose of human WNT4 is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for WNT4 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,052 Da
NCBI Official Full Name
protein Wnt-4
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 4
NCBI Official Symbol
WNT4
NCBI Official Synonym Symbols
WNT-4; SERKAL
NCBI Protein Information
protein Wnt-4
UniProt Protein Name
Protein Wnt-4
UniProt Gene Name
WNT4
UniProt Synonym Gene Names
UNQ426/PRO864
UniProt Entry Name
WNT4_HUMAN
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Product Notes
The Human WNT4 wnt4 (Catalog #AAA112849) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112849 ELISA Kit recognizes Human WNT4. It is sometimes possible for the material contained within the vial of "wingless-type MMTV integration site family, member 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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