Standard Curve (Sample)
Wingless-type MMTV integration site family, member 9B ELISA Kit | WNT9B elisa kit
Rat Wingless-type MMTV integration site family, member 9B ELISA Kit
Gene Names
WNT9B; WNT15; WNT14B
Reactivity
Rat
Synonyms
Wingless-type MMTV integration site family, member 9B; N/A; Rat Wingless-type MMTV integration site family, member 9B ELISA Kit; WNT9B elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of WNT9B. No significant cross-reactivity or interference between WNT9B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between WNT9B and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for WNT9B elisa kit
Intended Uses: This WNT9B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of RatWNT9B. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: WNT9B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-WNT9B antibody and an WNT9B-HRP conjugate. The assay sample and buffer are incubated together with WNT9B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the WNT9B concentration since WNT9B from samples and WNT9B-HRP conjugate compete for the anti-WNT9B antibody binding site. Since the number of sites is limited, as more sites are occupied by WNT9B from the sample, fewer sites are left to bind WNT9B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The WNT9B concentration in each sample is interpolated from this standard curve.
Principle of the Assay: WNT9B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-WNT9B antibody and an WNT9B-HRP conjugate. The assay sample and buffer are incubated together with WNT9B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the WNT9B concentration since WNT9B from samples and WNT9B-HRP conjugate compete for the anti-WNT9B antibody binding site. Since the number of sites is limited, as more sites are occupied by WNT9B from the sample, fewer sites are left to bind WNT9B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The WNT9B concentration in each sample is interpolated from this standard curve.
Product Categories/Family for WNT9B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,001 Da
NCBI Official Full Name
WNT9B
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 9B
NCBI Official Symbol
WNT9B
NCBI Official Synonym Symbols
WNT15; WNT14B
NCBI Protein Information
protein Wnt-9b; protein Wnt-14b; wingless-type MMTV integration site family, member 15
UniProt Protein Name
Protein Wnt-9b
UniProt Gene Name
WNT9B
UniProt Synonym Gene Names
WNT14B; WNT15; UNQ6973/PRO21956
UniProt Entry Name
WNT9B_HUMAN
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Product Notes
The Rat WNT9B wnt9b (Catalog #AAA94045) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA94045 ELISA Kit recognizes Rat WNT9B. It is sometimes possible for the material contained within the vial of "Wingless-type MMTV integration site family, member 9B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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