SDS-PAGE
(Figure 1. Human XAGE1A Protein, hFc Tag on SDS-PAGE under reducing condition.)
XAGE1A recombinant protein
Human XAGE1A Protein, hFc Tag
Gene Names
XAGE1B; CTP9; XAGE1; CT12.1; GAGED2; XAGE-1; XAGE1A; CT12.1A; CT12.1B
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
XAGE1A; N/A; Human XAGE1A Protein, hFc Tag; CT12.1; CT12.1A; CTP9; GAGED2; XAGE1; XAGE1A recombinant protein
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5 % to 8% trehalose is added as protectants before lyophilization.
Concentration
0.08mg/mL (varies by lot)
Reconstitution
1) Beofre opening the tube cap, centrifuge the sample tube at 5000g for 3-5 at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube.
2) Dissolved lyophilized protein sample in sterile water based on the recommended volume 625uL
3) After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
2) Dissolved lyophilized protein sample in sterile water based on the recommended volume 625uL
3) After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
Tag
N-Human Fc tag
Molecular Characterization
hFc(Glu99-Ala330) XAGE1A(Met1-Val81)
Molecular Weight Note
The protein has a predicted molecular mass of 35.2 kDa after removal of the signal peptide. The apparent molecular mass of hFc-XAGE1A is approximately 35-55 kDa due to glycosylation.
Visual Inspection
Test: Vial Label ; Specification: Label aligned and complete ; Result: Pass
Test: Bottle Sealing (manual check) ; Specification: Sealed tight ; Result: Pass
Test: Bottle Sealing (manual check) ; Specification: Sealed tight ; Result: Pass
Quantity Bioactivity
Test: BCA method ; Specification: > Specified quantity ; Result: Pass
Test: ELISA OD450 value (0.2ug/well) ; Specification: Bind to anti-DYKDDDDK monoclonal antibody ; Results: Pass
Test: ELISA OD450 value (0.2ug/well) ; Specification: Bind to anti-DYKDDDDK monoclonal antibody ; Results: Pass
Other Properties
Test: pH ; Specification: 8.0 ± 0.5 ; Result: Pass
Preparation and Storage
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).
SDS-PAGE
(Figure 1. Human XAGE1A Protein, hFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human XAGE1A Protein, hFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for XAGE1A recombinant protein
Description: Recombinant Human XAGE1A Protein with N-terminal human Fc tag
Background: This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene, in addition to alternative transcription start sites, results in multiple transcript variants. [provided by RefSeq, Jan 2010]
Background: This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene, in addition to alternative transcription start sites, results in multiple transcript variants. [provided by RefSeq, Jan 2010]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
X antigen family member 1 isoform a
NCBI Official Synonym Full Names
X antigen family, member 1B
NCBI Official Symbol
XAGE1B
NCBI Official Synonym Symbols
CTP9; XAGE1; CT12.1; GAGED2; XAGE-1; XAGE1A; CT12.1A; CT12.1B
NCBI Protein Information
X antigen family member 1; G antigen family D member 2; X antigen family, member 1A; cancer/testis antigen 12.1; cancer/testis antigen family 12, member 1a; cancer/testis antigen family 12, member 1b; cancer/testis associated protein; protein XAGE-1
UniProt Protein Name
X antigen family member 1
UniProt Gene Name
XAGE1A
UniProt Synonym Gene Names
GAGED2; XAGE1; XAGE-1; CT12.1
UniProt Entry Name
XAGE1_HUMAN
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Product Notes
The XAGE1A xage1a (Catalog #AAA47387) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "XAGE1A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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