Ku70 & Ku80 Heterodimer Recombinant Protein | XRCC5&XRCC6 recombinant protein

Ku70 & Ku80 Heterodimer Protein, Human, Recombinant (His Tag)

Cat. Num. AAA258267

• Gene Names: XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• Purity: ≥ 90 % as determined by SDS-PAGE
• Synonyms: Ku70 & Ku80 Heterodimer; N/A; Ku70 & Ku80 Heterodimer Protein, Human, Recombinant (His Tag); Human XRCC5 & XRCC6 Heterodimer Protein; KARP-1 Protein, Human; KARP1 Protein, Human; KU80 Protein, Human; Ku86 Protein, Human; KUB2 Protein, Human; NFIV Protein, Human; XRCC5 & XRCC6; XRCC5&XRCC6 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: ≥ 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

• Source: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His & His
• Protein Construction: A DNA sequence encoding the XRCC5 (P13010)(Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956)(Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
• Reconstitution: It is recommended that sterile water (200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV.
• Reconstitution: Reconstituted with sterile deionized water to 0.15 mg/mL.
• Molecular Mass: The recombinant heterodimer of human XRCC5/XRCC5 comprises 1379 (751 + 628) amino acids and has a calculated molecular mass of 157 (85 + 72) kDa. The apparent molecular mass of rh XRCC5/XRCC5 heterodimer is approximately 70 & 85 kDa respectively in SDS-PAGE under reducing conditions.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for XRCC5&XRCC6 recombinant protein

Background: X-ray repair cross-complementing protein 5, also known as 86kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 8kDa subunit, CTC box-binding factor 85kDa subunit, DNA repair protein XRCC5, Lupus Ku autoantigen protein p86, TLAA and XRCC5, is a nucleus and chromosome which belongs to the ku8 family. XRCC5 is a single-stranded DNA-dependent ATP-dependent helicase. XRCC5 has a role in chromosome translocation. X-ray repair cross-complementing protein 6, also known as 5'-deoxyribose-5-phosphate lyase Ku7, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 7kDa subunit, 7kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, CTC box-binding factor 75kDa subunit, Lupus Ku autoantigen protein p7, Thyroid-lupus autoantigen and XRCC6, is a nucleus and chromosome which belongs to the ku7 family. Heterodimer of a XRCC6 and a XRCC5 subunit associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NAA15, and this complex binds to the osteocalcin promoter and activates osteocalcin expression.

References

Featherstone C. et al., 1999, Mutat Res. 434: 3-15. Zhang Z. et al., 2001, J Biol Chem. 276: 38231-6. Hsu H-L. et al., 2002, DNA Repai.r 1: 225-35. Roberts SA. et al., 2010, Nature. 464:1214-7. Burkard TR. et al., 2011, BMC Syst Biol. 5: 17.

NCBI and Uniprot Product Information

• NCBI GI #: 10863945
• NCBI GeneID: 7520
• NCBI Accession #: NP_066964.1
• NCBI GenBank Nucleotide #: NM_021141.3
• UniProt Accession #: P13010; P12956
• Molecular Weight: Predicted Molecular Mass: 158.73 kDa
• NCBI Official Full Name: X-ray repair cross-complementing protein 5
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• NCBI Official Symbol: XRCC5
• NCBI Official Synonym Symbols: KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• NCBI Protein Information: X-ray repair cross-complementing protein 5; TLAA; CTC85; CTCBF; nuclear factor IV; Ku autoantigen, 80kDa; DNA repair protein XRCC5; thyroid-lupus autoantigen; 86 kDa subunit of Ku antigen; lupus Ku autoantigen protein p86; Ku86 autoantigen related protein
• UniProt Protein Name: X-ray repair cross-complementing protein 5
• UniProt Gene Name: XRCC5
• UniProt Synonym Gene Names: G22P2; CTC85; CTCBF; TLAA
• UniProt Entry Name: XRCC5_HUMAN