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product-image-AAA11830_SDS_PAGE.jpg SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

YBX1 recombinant protein

YBX1, 1-324aa, Human, His tag, E Coli

Gene Names
YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
YBX1; N/A; YBX1, 1-324aa, Human, His tag, E Coli; Nuclease-sensitive element-binding protein 1, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1; YBX1 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. Phosphate buffered saline (pH 7.4) containing 20% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE
Sequence Length
1-324aa
Applicable Applications for YBX1 recombinant protein
SDS-PAGE
Tag Information
His-tag
Domain
1-324aa
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

product-image-AAA11830_SDS_PAGE.jpg SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for YBX1 recombinant protein
YBX1, also known as Y box binding protein 1, associates with the selenocysteine insertion sequence and functions in mammalian selenoprotein translation. This protein is a potential drug target in cancer therapy. It helps the replication of adenovirus type 5, a commonly used vector in gene therapy. Thus, YBX1 can cause an oncolytic effect in YBX1 positive cancer cells treated with adenoviruses. Recombinant human YBX1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for YBX1 recombinant protein
References
Shen Q. et al. (2006) J Cell Physiol. 207:775-783.; Chernukhin IV. et al. (2000) J Biol Chem. 275:29915-29921

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38.3 kDa (347aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
nuclease-sensitive element-binding protein 1
NCBI Official Synonym Full Names
Y-box binding protein 1
NCBI Official Symbol
YBX1
NCBI Official Synonym Symbols
YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1
NCBI Protein Information
nuclease-sensitive element-binding protein 1
UniProt Protein Name
Nuclease-sensitive element-binding protein 1
UniProt Gene Name
YBX1
UniProt Synonym Gene Names
NSEP1; YB1; CBF-A; DBPB; EFI-A; YB-1

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Product Notes

The YBX1 ybx1 (Catalog #AAA11830) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's YBX1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the YBX1 ybx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE. It is sometimes possible for the material contained within the vial of "YBX1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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