Standard Curve (Sample)
Human Zinc Finger Homeobox Protein 1B ELISA Kit | ZFHX1B elisa kit
Human Zinc Finger Homeobox Protein 1B (ZFHX1B) (Sandwich ELISA) ELISA Kit
Gene Names
ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
Reactivity
Human
Synonyms
Zinc Finger Homeobox Protein 1B; N/A; Human Zinc Finger Homeobox Protein 1B (ZFHX1B) (Sandwich ELISA) ELISA Kit; ZEB2; SIP1; SIP-1; SMADIP1; Zinc Finger E-Box Binding Homeobox 2; SMAD Interacting Protein 1; ELISA Kit for Zinc Finger Homeobox Protein 1B (ZFHX1B); ZFHX1B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ZFHX1B. No significant cross-reactivity or interference between ZFHX1B and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.113ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ZFHX1B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ZFHX1B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ZFHX1B elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ZFHX1B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ZFHX1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ZFHX1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ZFHX1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ZFHX1B in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ZFHX1B in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Product Categories/Family for ZFHX1B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
136,447 Da
NCBI Official Full Name
zinc finger E-box-binding homeobox 2 isoform 2
NCBI Official Synonym Full Names
zinc finger E-box binding homeobox 2
NCBI Official Symbol
ZEB2
NCBI Official Synonym Symbols
SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
NCBI Protein Information
zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; SMAD interacting protein 1; Smad-interacting protein 1
UniProt Protein Name
Zinc finger E-box-binding homeobox 2
UniProt Gene Name
ZEB2
UniProt Synonym Gene Names
KIAA0569; SIP1; ZFHX1B; ZFX1B; SMADIP1
UniProt Entry Name
ZEB2_HUMAN
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Product Notes
The Human ZFHX1B zeb2 (Catalog #AAA189928) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189928 ELISA Kit recognizes Human ZFHX1B. It is sometimes possible for the material contained within the vial of "Zinc Finger Homeobox Protein 1B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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