Bioactivity
(carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5(Assay Buffer), initiated by addition 50 uL of various concentrations of CES1 (dilute by Assay Buffer) to 50 uL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37 degree C for 10min, then read at a wavelength of 400 nm.)
Carboxylesterase 1 (CES1) Active Protein | CES1 active protein
Active Carboxylesterase 1 (CES1)
Gene Names
CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Synonyms
Carboxylesterase 1 (CES1); N/A; Active Carboxylesterase 1 (CES1); CES1/Carboxylesterase 1; ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1; CES1 active protein
Host
293F cell
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Freeze-dried powder
Freeze-dried powder
Sequence
Gly18~Leu567
Applicable Applications for CES1 active protein
Activity Assay, Cell Culture
Species
Human
Isoelectric Point
6.5
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
Stable for 12 months if correctly stored.
Bioactivity
(carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5(Assay Buffer), initiated by addition 50 uL of various concentrations of CES1 (dilute by Assay Buffer) to 50 uL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37 degree C for 10min, then read at a wavelength of 400 nm.)
Bioactivity
(carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5(Assay Buffer), initiated by addition 50 uL of various concentrations of CES1 (dilute by Assay Buffer) to 50 uL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37 degree C for 10min, then read at a wavelength of 400 nm.)
SDS-PAGE
(SDS-PAGE)
SDS-PAGE
(SDS-PAGE)
Product Categories/Family for CES1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
567
NCBI Official Full Name
Liver carboxylesterase 1
NCBI Official Synonym Full Names
carboxylesterase 1
NCBI Official Symbol
CES1
NCBI Official Synonym Symbols
CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
NCBI Protein Information
liver carboxylesterase 1; egasyn; serine esterase 1; retinyl ester hydrolase; cocaine carboxylesterase; triacylglycerol hydrolase; carboxylesterase 2 (liver); brain carboxylesterase hBr1; cholesteryl ester hydrolase; monocyte/macrophage serine esterase; m
UniProt Protein Name
Liver carboxylesterase 1
UniProt Gene Name
CES1
UniProt Synonym Gene Names
CES2; SES1; ACAT; CE-1; hCE-1; REH; TGH
UniProt Entry Name
EST1_HUMAN
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Product Notes
The CES1 ces1 (Catalog #AAA152179) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Carboxylesterase 1 (CES1) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the CES1 ces1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gly18~Leu5 67. It is sometimes possible for the material contained within the vial of "Carboxylesterase 1 (CES1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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