Interleukin 10 (IL10) Active Protein | IL10 active protein

Active Interleukin 10 (IL10)

Cat. Num. AAA146605

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Applications: Activity Assay, Cell Culture
• Purity: > 97%
• Synonyms: Interleukin 10 (IL10); N/A; Active Interleukin 10 (IL10); CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor; IL10 active protein

• Host: 293F cell
• Purity/Purification: > 97%
• Form/Format: Freeze-dried powder
• Sequence Length: 178

• Applicable Applications for IL10 active protein: Activity Assay, Cell Culture
• Fragment: Ser19~Asn178
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Organism Species: Human
• Isoelectric Point: 7.6
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

SDS-PAGE

Activity

(Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4 T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) mediated induction of the pro-inflammatory cytokines TNFalpha, IL-1beta, IL-12, and IFNgamma made by cells such as macrophages and Th1 T cells. Therefore, THP1 cells were cultured in 24 well plates at a concentration of 106 cells/ml and activated by LPS (1ug/mL) in the absence or presence of IL-10 (5ng/mL, 10ng/mL) for 24h and production of IL-1beta was determined in the supernatants by cytokine specific ELISA.Activation of THP1 cells with LPS (1ug/mL) resulted in high levels of production of IL-1beta and strong inhibitory effects of IL-10 (5ng/mL, 10ng/mL) were observed on the production of IL-1beta as shown in Table 1.)

Product Categories/Family for IL10 active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 10835141
• NCBI GeneID: 3586
• NCBI Accession #: NP_000563.1
• NCBI GenBank Nucleotide #: NM_000572.2
• UniProt Accession #: P22301
• Molecular Weight: 20.3kDa; 18kDa
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10
• UniProt Protein Name: Interleukin-10
• UniProt Gene Name: IL10
• UniProt Synonym Gene Names: IL-10; CSIF