Interleukin 2 (IL2) Active Protein | IL2 active protein

Active Interleukin 2 (IL2)

Cat. Num. AAA146606

• Gene Names: Il2; Il-2
• Applications: Activity Assay, Cell Culture
• Purity: > 97%
• Synonyms: Interleukin 2 (IL2); N/A; Active Interleukin 2 (IL2); TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin; IL2 active protein

• Host: 293F cell
• Purity/Purification: > 97%
• Form/Format: Freeze-dried powder
• Sequence Length: 169

• Applicable Applications for IL2 active protein: Activity Assay, Cell Culture
• Fragment: Ala21~Gln169
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Organism Species: Mouse
• Isoelectric Point: 4.9
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

Application Data

Application Data

SDS-PAGE

Activity

(Interleukin-2 (IL-2), the main lymphokine produced by activated CD4 T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manner. Thus 3T3 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of IL2 for 48h, then 3T3 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.The inhibitory effect of IL-2 on cell proliferation of 3T3 cells observed by inverted microscope was shown in Figure 1.)

Product Categories/Family for IL2 active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 7110653
• NCBI GeneID: 16183
• NCBI Accession #: NP_032392.1
• NCBI GenBank Nucleotide #: NM_008366.3
• UniProt Accession #: P04351; P97945; Q791T3
• Molecular Weight: 18.9kDa; 25kDa
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: Il2
• NCBI Official Synonym Symbols: Il-2
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: Interleukin-2
• UniProt Gene Name: Il2
• UniProt Synonym Gene Names: Il-2; IL-2; TCGF