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WB (Western Blot)
(Western BlotSample: Recombinant HRG, Human;Antibody: Rabbit Anti-Human HRG Ab)
Histidine Rich Glycoprotein (HRG) Active Protein | HRG active protein
Active Histidine Rich Glycoprotein (HRG)
Gene Names
HRG; HPRG; HRGP; THPH11
Reactivity
Homo sapiens (Human)
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>98%
Synonyms
Histidine Rich Glycoprotein (HRG); N/A; Active Histidine Rich Glycoprotein (HRG); HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG; HRG active protein
Host
293F cell
Reactivity
Homo sapiens (Human)
Purity/Purification
>98%
Form/Format
PBS, pH7.6, containing 5% trehalose.
Applicable Applications for HRG active protein
Invitro Assay, Activity Assay, Cell Culture
Source
Eukaryotic expression
Residues
Val19~Lys525
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted isoelectric point
7.0
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western BlotSample: Recombinant HRG, Human;Antibody: Rabbit Anti-Human HRG Ab)
WB (Western Blot)
(Western BlotSample: Recombinant HRG, Human;Antibody: Rabbit Anti-Human HRG Ab)
SDS_PAGE
(Sample: Active recombinant HRG, Human)
SDS_PAGE
(Sample: Active recombinant HRG, Human)
Gene Sequencing
(Gene Sequencing (extract))
Gene Sequencing
(Gene Sequencing (extract))
Bioactivity
(HRG (Histidine-rich glycoprotein) is a plasma glycoprotein which is distinguished by its high content of histidine and proline. HRG binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Thus, a functional binding ELISA assay was constructed to detect the association of rhHRG with heparin. Briefly, Microtiter wells were coated with OVA-conjugated-heparin. rhHRG were diluted serially in 0.01M PBS (pH 7.4). Duplicate samples of 100uL were then transferred to the coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-HRG PAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate Solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately.The binding activity of rhHRG with heparin was shown in Figure 1 and this effect was in a dose dependent manner.)
Bioactivity
(HRG (Histidine-rich glycoprotein) is a plasma glycoprotein which is distinguished by its high content of histidine and proline. HRG binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Thus, a functional binding ELISA assay was constructed to detect the association of rhHRG with heparin. Briefly, Microtiter wells were coated with OVA-conjugated-heparin. rhHRG were diluted serially in 0.01M PBS (pH 7.4). Duplicate samples of 100uL were then transferred to the coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-HRG PAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate Solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately.The binding activity of rhHRG with heparin was shown in Figure 1 and this effect was in a dose dependent manner.)
Sequence
Sequence
Product Categories/Family for HRG active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 59.3kDa
Accurate Molecular Mass: 75kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 75kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
histidine-rich glycoprotein
NCBI Official Synonym Full Names
histidine rich glycoprotein
NCBI Official Symbol
HRG
NCBI Official Synonym Symbols
HPRG; HRGP; THPH11
NCBI Protein Information
histidine-rich glycoprotein
UniProt Protein Name
Histidine-rich glycoprotein
UniProt Gene Name
HRG
UniProt Synonym Gene Names
HPRG
Similar Products
Product Notes
The HRG hrg (Catalog #AAA146623) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. The Active Histidine Rich Glycoprotein (HRG) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Histidine Rich Glycoprotein (HRG) can be used in a range of immunoassay formats including, but not limited to, Invitro Assay, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the HRG hrg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histidine Rich Glycoprotein (HRG), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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