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IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using HSPA9 antibody at dilution of 1:100 (40x lens).)
Rabbit HSPA9 Polyclonal Antibody | anti-HSPA9 antibody
HSPA9 Polyclonal Antibody
Gene Names
HSPA9; CSA; MOT; MOT2; CRP40; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunoprecipitation
Purity
Affinity purification
Synonyms
HSPA9; Polyclonal Antibody; HSPA9 Polyclonal Antibody; CRP40;CSA;EVPLS;GRP-75;GRP75;HELS-124m;HSPA9B;MOT;MOT2;MTHSP75;PBP74;SAAN;SIDBA4; anti-HSPA9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Concentration
1mg/ml (varies by lot)
Sequence Length
679
Applicable Applications for anti-HSPA9 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), RIP
Application Notes
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Source
Rabbit
Immunogen
Recombinant protein of human HSPA9
Observed MW
80kDa
Calculated MW
73kDa
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat ovary using HSPA9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat ovary using HSPA9 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HSPA9 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HSPA9 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-HSPA9 antibody
Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).
Product Categories/Family for anti-HSPA9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
679
NCBI Official Full Name
Stress-70 protein, mitochondrial
NCBI Official Synonym Full Names
heat shock 70kDa protein 9 (mortalin)
NCBI Official Symbol
HSPA9
NCBI Official Synonym Symbols
CSA; MOT; MOT2; CRP40; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
NCBI Protein Information
stress-70 protein, mitochondrial; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m
UniProt Protein Name
Stress-70 protein, mitochondrial
UniProt Gene Name
HSPA9
UniProt Synonym Gene Names
GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
UniProt Entry Name
GRP75_HUMAN
Similar Products
Product Notes
The HSPA9 hspa9 (Catalog #AAA10628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPA9 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), RIP. WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200. Researchers should empirically determine the suitability of the HSPA9 hspa9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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