Matrix Metalloproteinase-3 Active Protein | MMP 3 active protein
Recombinant Human Matrix Metalloproteinase-3, HEK
Gene Names
MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Matrix Metalloproteinase-3; Recombinant Human Matrix Metalloproteinase-3; HEK; MMP 3 Human; Matrix Metalloproteinase-3 Human Recombinant; Stromelysin-1; EC 3.4.24.17; Matrix metalloproteinase-3; MMP-3; Transin-1; SL-1; STMY; STR1; STMY1; MGC126102; MGC126103; MGC126104; MMP 3 HEK; MMP 3 active protein
Host
HEK293 Cells
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
The MMP-3 (0.30mg/ml) is supplied as a 0.2 um filtered solution in Tris, NaCl and Brij35.
Physical Appearance: Sterile Filtered colorless solution.
Physical Appearance: Sterile Filtered colorless solution.
Concentration
0.30mg/ml (varies by lot)
Sequence Length
477
Biological Activity
The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 150 pmoles/min/ ug.Recombinant Human MMP-3 protein pro form needs to be activated with Chymotrypsin.Activation Protocol:1. Dilute MMP3 to 20 ug/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP3 by adding Chymotrypsin(Sigma, Catalog#C3142,1mg/ml stock in 1mM HCl) to a final concentration of 5ug/ml.3. Incubate at 37 degree C for 30 minutes.4. Stop activation with 2mM PMSF. Pre-warm the PMSF to 37 degree C prior to adding to sample.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.Avoid multiple freeze-thaw cycles.
Related Product Information for MMP 3 active protein
Description: MMP-3 Human Recombinant produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-3 is purified by proprietary chromatographic techniques.
Introduction: MMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk. MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.
Introduction: MMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk. MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.
Product Categories/Family for MMP 3 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
stromelysin-1 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 3
NCBI Official Symbol
MMP3
NCBI Official Synonym Symbols
SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
NCBI Protein Information
stromelysin-1; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); matrix metalloproteinase-3; proteoglycanase; transin-1
UniProt Protein Name
Stromelysin-1
UniProt Gene Name
MMP3
UniProt Synonym Gene Names
STMY1; SL-1; MMP-3
UniProt Entry Name
MMP3_HUMAN
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Product Notes
The MMP 3 mmp3 (Catalog #AAA10817) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase-3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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