Rat Choline acetyltransferase, ChAT ELISA Kit | ChAT elisa kit
Rat Choline acetyltransferase, ChAT ELISA Kit
Gene Names
CHAT; CMS6; CMS1A; CMS1A2; CHOACTASE
Reactivity
Rat
Synonyms
Choline acetyltransferase, ChAT; N/A; Rat Choline acetyltransferase, ChAT ELISA Kit; ChAT elisa kit
Reactivity
Rat
Sequence Length
95
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05mmol/L-30mmol/L
Sensitivity
0.02mmol/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for ChAT elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Cholesterol (also known as CH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CH antibody. CH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CH Antibody is added and binds to CH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CH antibody. CH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CH Antibody is added and binds to CH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
74,361 Da
NCBI Official Full Name
choline acetyltransferase, partial
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS6; CMS1A; CMS1A2; CHOACTASE
NCBI Protein Information
choline O-acetyltransferase
UniProt Protein Name
Choline O-acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
