Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online Need help? +1-800-604-9114 or contact us
Cart Login
product-image-AAA16189_SC.jpg
Standard Curve (Sample)

Mouse Choline acetyltransferase ELISA Kit | ChAT elisa kit

Mouse Choline acetyltransferase ELISA Kit

Gene Names
CHAT; CMS1A; CMS1A2; CHOACTASE
Reactivity
Mouse
Synonyms
Choline acetyltransferase; N/A; Mouse Choline acetyltransferase ELISA Kit; ChAT elisa kit
Ordering
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of ChAT. No significant cross-reactivity or interference between ChAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ChAT and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ChAT elisa kit
Principle of the Assay: ChAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ChAT antibody and an ChAT-HRP conjugate. The assay sample and buffer are incubated together with ChAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ChAT concentration since ChAT from samples and ChAT-HRP conjugate compete for the anti-ChAT antibody binding site. Since the number of sites is limited, as more sites are occupied by ChAT from the sample, fewer sites are left to bind ChAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ChAT concentration in each sample is interpolated from this standard curve.

Intended Uses: This ChAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ChAT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for ChAT elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
70,394 Da
NCBI Official Full Name
choline acetyltransferase, partial
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS1A; CMS1A2; CHOACTASE
NCBI Protein Information
choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase
UniProt Protein Name
Choline O-acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
UniProt Entry Name
CLAT_HUMAN

Similar Products

product-image-AAA22102_SC.jpg

CHAT Assay Kit

Host: n/a
Reactivity: n/a
Apps: n/a
product-image-AAA11137_SC.jpg

ChAT ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA16189_SC.jpg

ChAT ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA17759_SC.jpg

CHAT ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA30341_FCM6.jpg

Choline Acetyltransferase Antibody

Host: Rabbit
Reactivity: Human, Mouse, Rat
Apps: Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
product-image-AAA18573_SDS_PAGE.png

bar Recombinant Protein

Host: E Coli or Yeast or Baculovirus or Mammalian Cell
Reactivity: n/a
Apps: n/a
product-image-AAA18616_SDS_PAGE.png

pac Recombinant Protein

Host: E Coli or Yeast or Baculovirus or Mammalian Cell
Reactivity: n/a
Apps: n/a
product-image-AAA27173_SC.jpg

C6orf134 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA18776_SDS_PAGE.jpg

pat Recombinant Protein

Host: E Coli or Yeast or Baculovirus or Mammalian Cell
Reactivity: n/a
Apps: n/a
product-image-AAA20633_SC.jpg

SAT1 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA21022_IHC2.jpg

NAT8L Antibody

Host: Rabbit
Reactivity: Homo sapiens (Human)
Apps: Western Blot
product-image-AAA27118_SC.jpg

SLC44A1 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA15215_SC.png

ANG- ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA18215_SC.png

ANG ELISA Kit

Host: n/a
Reactivity: Pig
Apps: n/a
product-image-AAA21633_SC.jpg

Ang-II ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA22445_SC.jpg

ANG-1 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA17627_SC.jpg

ANG II ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA15491_SC.png

ANG-II ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA12606_SC.jpg

Ang-I ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a

Product Notes

The Mouse ChAT chat (Catalog #AAA16189) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA16189 ELISA Kit recognizes Mouse ChAT. It is sometimes possible for the material contained within the vial of "Choline acetyltransferase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.