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IHC (Immunohistchemistry)
(Anti-DDX4/MVH antibody, AAA11583, IHC(F)IHC(F): Rat Intestine Tissue)
Rabbit DDX4/MVH Polyclonal Antibody | anti-DDX4 antibody
Anti-DDX4/MVH antibody
Gene Names
DDX4; VASA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Synonyms
DDX4/MVH; Polyclonal Antibody; Anti-DDX4/MVH antibody; Probable ATP-dependent RNA helicase DDX4; DEAD(Asp-Glu-Ala-Asp) box polypeptide 4; DDX 4 antibody; Ddx4 antibody; DDX4_HUMAN antibody; DEAD(Asp Glu Ala Asp) box polypeptide 4 antibody; DEAD box protein 4 antibody; Dead-box protein 4 antibody; DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody; MGC111074 antibody; MVH antibody; Probable ATP dependent RNA helicase DDX4 antibody; Probable ATP-dependent RNA helicase DDX4 antibody; VASA antibody; VASA homolog antibody; anti-DDX4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
724
Applicable Applications for anti-DDX4 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human DDX4 (253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistchemistry)
(Anti-DDX4/MVH antibody, AAA11583, IHC(F)IHC(F): Rat Intestine Tissue)
IHC (Immunohistchemistry)
(Anti-DDX4/MVH antibody, AAA11583, IHC(F)IHC(F): Rat Intestine Tissue)
ICC (Immunocytochemistry)
(Anti-DDX4/MVH antibody, AAA11583, ICCICC: MCF-7 Cell)
ICC (Immunocytochemistry)
(Anti-DDX4/MVH antibody, AAA11583, ICCICC: MCF-7 Cell)
IHC (Immunohistochemistry)
(Anti-DDX4/MVH antibody, AAA11583, IHC(P)IHC(P): Rat Testis Tissue)
IHC (Immunohistochemistry)
(Anti-DDX4/MVH antibody, AAA11583, IHC(P)IHC(P): Rat Testis Tissue)
IHC (Immunohistochemistry)
(Anti-DDX4/MVH antibody, AAA11583, IHC(P)IHC(P): Rat Ovary Tissue)
IHC (Immunohistochemistry)
(Anti-DDX4/MVH antibody, AAA11583, IHC(P)IHC(P): Rat Ovary Tissue)
WB (Western Blot)
(Anti-DDX4/MVH antibody, AAA11583, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: Mouse Ovary Tissue Lysate)
WB (Western Blot)
(Anti-DDX4/MVH antibody, AAA11583, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: Mouse Ovary Tissue Lysate)
WB (Western Blot)
(Anti-DDX4/MVH antibody, AAA11583, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: HELA Cell Lysate)
WB (Western Blot)
(Anti-DDX4/MVH antibody, AAA11583, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: HELA Cell Lysate)
Related Product Information for anti-DDX4 antibody
Description: Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX4(DDX4) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
Background: DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
77,056 Da
NCBI Official Full Name
Probable ATP-dependent RNA helicase DDX4
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
NCBI Official Symbol
DDX4
NCBI Official Synonym Symbols
VASA
NCBI Protein Information
probable ATP-dependent RNA helicase DDX4; vasa homolog; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX4
UniProt Gene Name
DDX4
UniProt Synonym Gene Names
VASA
UniProt Entry Name
DDX4_HUMAN
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Product Notes
The DDX4 ddx4 (Catalog #AAA11583) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DDX4/MVH antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DDX4/MVH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC. Researchers should empirically determine the suitability of the DDX4 ddx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX4/MVH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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