FCM (Flow Cytometry)
(Flow cytometry analysis of Hsp105alpha in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
Mouse anti-Human HSP105alpha Monoclonal Antibody | anti-HSP105alpha antibody
HSP105alpha antibody
Gene Names
HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
HSP105alpha; Monoclonal Antibody; HSP105alpha antibody; Heat shock 105kDa/110kDa protein 1; HSPH1; HSP105-alpha; Heat shock 105kDa/110kDa protein 1 Antigen NY CO 25; DKFZp686M05240; Heat shock 105kD; Heat shock 105kD alpha; Heat shock 105kD beta; Heat shock 105kDa protein; Heat shock 105kDa protein 1; Heat shock 110kDa protein; Heat shock protein 105 kDa; HSP105A; HSP105B; HSP110; HSPH 1; KIAA0201; NY CO 25.; anti-HSP105alpha antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b heavy chain and kappa light chain
Clone Number
J1G12
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
858
Applicable Applications for anti-HSP105alpha antibody
ELISA, WB, Flow cytometry, ICC/IF
Application Notes
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Immunogen
Recombinant human Hsp105 alpha (1-858aa) purified from E. coli
Clone
Anti-human Hsp105 ? mAb, clone J1G12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp105 ? protein.
Clone Note
Anti-human Hsp105 alpha mAb, clone J1G12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp105 alpha protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
FCM (Flow Cytometry)
(Flow cytometry analysis of Hsp105alpha in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
FCM (Flow Cytometry)
(Flow cytometry analysis of Hsp105alpha in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
ICC (Immunocytochemistry)
(ICC/IF analysis of Hsp105alpha in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Hsp105alpha antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
ICC (Immunocytochemistry)
(ICC/IF analysis of Hsp105alpha in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Hsp105alpha antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp105 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysateLane 3.: NIH-3T3 cell lysateLane 4.: MCF7 cell lysateLane 5.: U-87 MG cell lysateLane 6.: A549 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp105 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysateLane 3.: NIH-3T3 cell lysateLane 4.: MCF7 cell lysateLane 5.: U-87 MG cell lysateLane 6.: A549 cell lysate)
Related Product Information for anti-HSP105alpha antibody
Hsp105 is a mammalian member of the HSP105/110 family, a diverged subgroup of the HSP70 family. HSP105 exists as two isoforms, alpha and beta. Hsp105 alpha associates with Hsp70/Hsc70 as complexes in vivo and regulates the chaperone activity of Hsp70/Hsc70 negatively in vitro and in vivo. Hsp105 alpha is constitutively expressed and also induced by various stress, whereas Hsp105beta, lacking 44 amino acids from Hsp105 alpha, is specifically expressed during mild heat shock. Although Hsp105 alpha is shown to localize in the cytoplasm of mammalian cells, cellular localization of Hsp105beta is not known
Product Categories/Family for anti-HSP105alpha antibody
References
Wang XY., et al. (2001) J Immunol. 166 (1):490-7.; Yamagishi N., et al. (2006) Exp Cell Res. 312 (17):3215-23. ;Saito Y., et al. (2007) Exp Cell Res. 313 (17):3707-17.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,460 Da
NCBI Official Full Name
heat shock protein 105 kDa isoform 1
NCBI Official Synonym Full Names
heat shock protein family H (Hsp110) member 1
NCBI Official Symbol
HSPH1
NCBI Official Synonym Symbols
HSP105; HSP105A; HSP105B; NY-CO-25
NCBI Protein Information
heat shock protein 105 kDa
UniProt Protein Name
Heat shock protein 105 kDa
UniProt Gene Name
HSPH1
UniProt Synonym Gene Names
HSP105; HSP110; KIAA0201
Similar Products
Product Notes
The HSP105alpha hsph1 (Catalog #AAA11727) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP105alpha antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSP105alpha can be used in a range of immunoassay formats including, but not limited to, ELISA, WB, Flow cytometry, ICC/IF. The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the HSP105alpha hsph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP105alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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