Human Heat Shock Protein 27 (Hsp27) ELISA Kit | Hsp27 elisa kit
Human Heat Shock Protein 27 (Hsp27) ELISA Kit
Reactivity
Human
Synonyms
Heat Shock Protein 27 (Hsp27); N/A; Human Heat Shock Protein 27 (Hsp27) ELISA Kit; Hsp27 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Hsp27. No significant cross-reactivity or interference between Hsp27 and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.059ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hsp27 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hsp27 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Standard Curve (Sample)
Related Product Information for Hsp27 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Hsp27 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Hsp27. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hsp27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hsp27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hsp27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Hsp27. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hsp27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hsp27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hsp27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
