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Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Med power)
Mouse CD43 Monoclonal Antibody | anti-CD43 antibody
MOUSE ANTI RAT CD43:RPE
Gene Names
Spn; Lsn; Cd43; Lsn1; gpL115
Applications
Flow Cytometry, Functional Assay
Synonyms
CD43, Antibody; MOUSE ANTI RAT CD43:RPE; anti-CD43 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
W3/13
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Sequence Length
378
Applicable Applications for anti-CD43 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Flow Cytometry: Maximum Dilution: Neat
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers
Perservative Stabilisers
Immunogen
Rat thymocyte membrane glycoproteins
Fusion Partners
Fusion Partners
Target Species
Rat
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Med power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Med power)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. High power)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. High power)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD43: FITC)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD43: FITC)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. High power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. High power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Low power)
Related Product Information for anti-CD43 antibody
Mouse anti Rat CD43 antibody, clone W3/13 recognizes the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmebrane glycoprotein (Killeen et al. 1987) expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions (van den Berg et al. 2001) Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
38,426 Da
NCBI Official Full Name
Leukosialin
NCBI Official Synonym Full Names
sialophorin
NCBI Official Symbol
Spn
NCBI Official Synonym Symbols
Lsn; Cd43; Lsn1; gpL115
NCBI Protein Information
leukosialin; W3/13 antigen; leukocyte sialoglycoprotein; leukosianin
UniProt Protein Name
Leukosialin
UniProt Gene Name
Spn
UniProt Entry Name
LEUK_RAT
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Product Notes
The CD43 spn (Catalog #AAA12052) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD43 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD43 spn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD43, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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