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Standard Curve (Sample)

Rat 5-hydroxy tryptamine (5-ht) ELISA Kit | 5-ht elisa kit

Rat 5-hydroxy tryptamine (5-ht) ELISA Kit

Gene Names
HTR1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
Reactivity
Rat
Synonyms
5-hydroxy tryptamine (5-ht); N/A; Rat 5-hydroxy tryptamine (5-ht) ELISA Kit; 5-ht elisa kit
Ordering
 
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For Research Use Only!
Reactivity
Rat
Sequence Length
422
Samples
Rat serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
100 ng/mL-1.56 ng/mL
Sensitivity
Up to 0.5 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for 5-ht elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat 5-ht monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
46,107 Da
NCBI Official Full Name
5-hydroxytryptamine receptor 1A
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled
NCBI Official Symbol
HTR1A
NCBI Official Synonym Symbols
G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
NCBI Protein Information
5-hydroxytryptamine receptor 1A; 5-HT1a receptor; guanine nucleotide-binding regulatory protein-coupled receptor
UniProt Protein Name
5-hydroxytryptamine (Serotonin) receptor 1A
UniProt Gene Name
5HT1A
UniProt Synonym Gene Names
HTR1A; hCG_16836
UniProt Entry Name
Q5ZGX3_HUMAN

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Product Notes

The Rat 5-ht 5ht1a (Catalog #AAA12908) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA12908 ELISA Kit recognizes Rat 5-ht. It is sometimes possible for the material contained within the vial of "5-hydroxy tryptamine (5-ht), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.